Department of Environmental Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea.
J Microbiol Biotechnol. 2023 Apr 28;33(4):471-484. doi: 10.4014/jmb.2210.10038. Epub 2023 Feb 10.
Compost is widely used as an organic additive to improve the bioremediation of diesel-contaminated soil. In this study, the effects of compost amendment on the remediation performance, functional genes, and bacterial community are evaluated during the bioremediation of diesel-contaminated soils with various ratios of compost (0-20%, w/w). The study reveals that the diesel removal efficiency, soil enzyme (dehydrogenase and urease) activity, soil CH oxidation potential, and soil NO reduction potential have a positive correlation with the compost amendment ( < 0.05). The ratios of denitrifying genes (, and ) to 16S rRNA genes each show a positive correlation with compost amendment, whereas the ratio of the CH-oxidizing gene () to the 16S rRNA genes shows a negative correlation. Interestingly, the genera , , , , , , , and are strongly associated with diesel degradation, and have a strong positive correlation with soil CH oxidation potential. Meanwhile, the genera , , , , , , and are found to be strongly associated with soil NO reduction potential. These results provide useful data for developing technologies that improve diesel removal efficiency while minimizing greenhouse gas emissions in the bioremediation process of diesel-contaminated soil.
堆肥广泛用作有机添加剂，以提高受柴油污染土壤的生物修复效果。在这项研究中，评估了在不同比例（0-20%，w/w）的堆肥添加条件下，堆肥添加对受柴油污染土壤的生物修复性能、功能基因和细菌群落的影响。研究表明，柴油去除效率、土壤酶（脱氢酶和脲酶）活性、土壤 CH 氧化潜能和土壤 NO 还原潜能与堆肥添加呈正相关（<0.05）。反硝化基因（、和）与 16S rRNA 基因的比值与堆肥添加呈正相关，而 CH 氧化基因（）与 16S rRNA 基因的比值呈负相关。有趣的是，属、、、、、、和与柴油降解密切相关，与土壤 CH 氧化潜能呈强正相关。同时，属、、、、、、和与土壤 NO 还原潜能密切相关。这些结果为开发技术提供了有用的数据，这些技术可以在受柴油污染土壤的生物修复过程中提高柴油去除效率，同时最大限度地减少温室气体排放。
