Department of Mechanics, Politecnico di Torino, Turin, Italy.
Int J Biol Macromol. 2011 Nov 1;49(4):700-6. doi: 10.1016/j.ijbiomac.2011.07.002. Epub 2011 Jul 13.
Scaffolds based on gelatin (G) are considered promising for tissue engineering, able to mimic the natural extracellular matrix. G drawback is its poor structural consistency in wet conditions. Therefore, crosslinking is necessary to fabricate stable G scaffolds. In this work, a comparative study between the performance of two different crosslinkers, genipin (GP) and γ-glycidoxypropyltrimethoxysilane (GPTMS), is presented. Flat membranes by solvent casting and porous crosslinked scaffolds by freeze-drying were prepared. Infrared spectroscopy and thermal analysis were applied to confirm G chain crosslinking. Moreover, GP and GPTMS increased the stability of G in aqueous media and improved the mechanical properties. Crosslinking reduced the wettability, especially in the case of G_GPTMS samples, due to the introduction of hydrophobic siloxane chains. Both G_GP and G_GPTMS scaffolds supported MG-63 osteoblast-like cell adhesion and proliferation.
基于明胶（G）的支架被认为是有前途的组织工程材料，能够模拟天然细胞外基质。G 的缺点是在潮湿条件下结构一致性差。因此，需要交联来制造稳定的 G 支架。在这项工作中，比较了两种不同交联剂——京尼平（GP）和γ-缩水甘油丙基三甲氧基硅烷（GPTMS）的性能。通过溶剂浇铸制备了平面膜，通过冷冻干燥制备了多孔交联支架。应用红外光谱和热分析来证实 G 链的交联。此外，GP 和 GPTMS 提高了 G 在水介质中的稳定性，并改善了机械性能。交联降低了亲水性，特别是在 G_GPTMS 样品的情况下，这是由于引入了疏水性硅氧烷链。G_GP 和 G_GPTMS 支架均支持 MG-63 成骨样细胞的黏附和增殖。
