Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China.
J Tissue Eng Regen Med. 2011 Feb;5(2):156-62. doi: 10.1002/term.301.
This study evaluates the feasibility of poly(ε-caprolactone) as a tracheal replacement. To improve biocompatibility, the lumen was modified by gelatin hydrogel crosslinked with two different reagents, EDC and genipin. It was found that the choice of crosslinking agents could significantly affect human lung carcinoma cell proliferation. Genipin-crosslinked gelatin hydrogel had significantly better cell proliferation than EDC-crosslinked hydrogel. The study further investigated the performance of the PCL tube modified by genipin-crosslinked gelatin, using a rabbit tracheal implantation model with implants harvested and histologically examined. In vivo results showed that the PCL tube possessed suitable mechanical properties for resisting collapse during implantation. Additionally, PCL modified by genipin-crosslinked gelatin was found to suppress granulation tissue growth and prolong animal survival time in comparison with the original PCL tube. Genipin could be an effective treatment to reduce granulation tissue formation at the tracheal anastomoses.
本研究评估了聚己内酯（PCL）作为气管替代物的可行性。为了提高生物相容性，通过两种不同试剂（EDC 和京尼平）交联明胶水凝胶来修饰管腔。结果发现，交联剂的选择会显著影响人肺癌细胞的增殖。与 EDC 交联水凝胶相比，京尼平交联明胶水凝胶具有更好的细胞增殖效果。该研究进一步通过使用兔气管植入模型，对经京尼平交联明胶修饰的 PCL 管的性能进行了研究，植入物被取出并进行组织学检查。体内结果表明，PCL 管具有合适的机械性能，可以在植入过程中抵抗塌陷。此外，与原始 PCL 管相比，用京尼平交联明胶修饰的 PCL 被发现可以抑制肉芽组织的生长并延长动物的存活时间。京尼平可能是一种有效减少气管吻合处肉芽组织形成的治疗方法。
