Department of Biochemistry and Molecular Biology, Anhui Medical University, 69 Meishan Road, Hefei, 230032, Anhui, China.
Mol Biol Rep. 2012 Apr;39(4):4201-5. doi: 10.1007/s11033-011-1205-4. Epub 2011 Jul 17.
Interleukin (IL)-15 is an important inflammatory cytokine and plays a key role in autoimmune disease. At present, IL-15 gene expression and regulation related to many innate immunity trigger signals have been clarified in some specific cell types, but the relationship of IL-6 and IL-15 in the human keratinocyte cell line (HaCaT) is unknown. In this study, we investigated the effect of IL-6 on the expression of IL-15 and selected signaling pathways in HaCaT cells. Results demonstrated that IL-6 up-regulated the expression of IL-15 both at the mRNA and protein levels. Meanwhile, IL-6 was able to activate MAPKs-ERK1/2 and PI3K-AKT signaling pathways. Furthermore, the high expression of IL-15 induced by IL-6 was down-regulated while MAPKs-ERK1/2 and PI3K-AKT signaling pathways were, respectively, blocked by PD98059 and LY294002. These findings indicate that the expression of IL-15 up-regulated by IL-6 is associated with MAPKs-ERK1/2 and PI3K-AKT signaling pathways in HaCaT cells.
白细胞介素 (IL)-15 是一种重要的炎症细胞因子，在自身免疫性疾病中发挥关键作用。目前，在某些特定的细胞类型中，已经阐明了与许多先天免疫触发信号相关的 IL-15 基因表达和调节，但人角质形成细胞系 (HaCaT) 中 IL-6 和 IL-15 之间的关系尚不清楚。在本研究中，我们研究了 IL-6 对 HaCaT 细胞中 IL-15 表达和选择信号通路的影响。结果表明，IL-6 可在上、下调 IL-15 的 mRNA 和蛋白水平。同时，IL-6 能够激活 MAPKs-ERK1/2 和 PI3K-AKT 信号通路。此外，由 IL-6 诱导的 IL-15 高表达被 PD98059 和 LY294002 分别阻断 MAPKs-ERK1/2 和 PI3K-AKT 信号通路后下调。这些发现表明，IL-6 上调的 IL-15 表达与 HaCaT 细胞中的 MAPKs-ERK1/2 和 PI3K-AKT 信号通路有关。
