Celestino-Santos W, Bezerra A G Júnior, Cezar A B, Mattoso N, Schreiner W H
Departamento de Física, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, 81531-880 Curitiba, Paraná, Brazil.
J Nanosci Nanotechnol. 2011 Jun;11(6):4702-7. doi: 10.1166/jnn.2011.4194.
We report on the synthesis of vanadium oxide nanoparticles using the laser ablation in solution technique. The particles were characterized by dynamic light scattering-DLS, transmission electron microscopy-TEM, X-ray diffraction-XRD, X-ray photoelectron spectroscopy-XPS and UV-Vis optical spectroscopy. The oxide nanoparticles are mainly composed of tetragonal V2O5, a semiconductor with a 2.2 eV band gap. The interaction of the nanoparticles with cysteine, a very important aminoacid present in proteins, was studied. Upon reaction with cysteine, the bandgap of the nanoparticles shifts to the ultraviolet region at 2.87 eV. This color change from yellow to transparent can be used for selective cysteine sensing. Additionally, the intervalence band of the optical absorption spectra shows capability for cysteine sensing in the microM range.
我们报道了使用溶液中的激光烧蚀技术合成氧化钒纳米颗粒。通过动态光散射（DLS）、透射电子显微镜（TEM）、X射线衍射（XRD）、X射线光电子能谱（XPS）和紫外可见光谱对这些颗粒进行了表征。氧化物纳米颗粒主要由四方相的V2O5组成，V2O5是一种带隙为2.2 eV的半导体。研究了纳米颗粒与蛋白质中一种非常重要的氨基酸——半胱氨酸的相互作用。与半胱氨酸反应后，纳米颗粒的带隙移至2.87 eV的紫外区域。这种从黄色到透明的颜色变化可用于选择性半胱氨酸传感。此外，光吸收光谱的价带间显示出在微摩尔范围内对半胱氨酸传感的能力。
