Akin H E, Karabay D A O, Kyle J R, Mills A P, Ozkan C S, Ozkan M
Electrical Engineering Department, University of California, Riverside, CA 92521, USA.
J Nanosci Nanotechnol. 2011 Jun;11(6):4717-23. doi: 10.1166/jnn.2011.38844717.
DNA Computing is a rapidly-developing interdisciplinary area which could benefit from more experimental results to solve practical problems with the current biological tools. In this study, we have integrated microelectronics and molecular biology techniques for the storage of information and basic arithmetic operations via DNA. Using 16 different complementary sequences of DNA, we stored 4 bits of information on an electronic microarray and read the data via the fluorescent signal strength coming from the microarray pads. We also showed the possibility of addition and subtraction of quantities of fluorescently tagged DNA determined via their fluorescent signal strength. We conclude that the hybrid technology we employed, based on a matured Si-CMOS platform, has the potential to strengthen the pursuit of DNA Computation as well as finding its own niche applications.
DNA计算是一个快速发展的跨学科领域，它可以从更多的实验结果中受益，以便利用当前的生物工具解决实际问题。在本研究中，我们整合了微电子学和分子生物学技术，用于通过DNA进行信息存储和基本算术运算。我们使用16种不同的DNA互补序列，在电子微阵列上存储了4位信息，并通过来自微阵列垫的荧光信号强度读取数据。我们还展示了通过荧光信号强度确定的荧光标记DNA数量进行加减法运算的可能性。我们得出结论，我们采用的基于成熟的硅互补金属氧化物半导体（Si-CMOS）平台的混合技术，有潜力加强对DNA计算的探索，并找到其自身的特定应用领域。
