Song Youngjun, Kim Sejung, Heller Michael J, Huang Xiaohua
Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Nat Commun. 2018 Jan 18;9(1):281. doi: 10.1038/s41467-017-02705-8.
DNA has been employed to either store digital information or to perform parallel molecular computing. Relatively unexplored is the ability to combine DNA-based memory and logical operations in a single platform. Here, we show a DNA tri-level cell non-volatile memory system capable of parallel random-access writing of memory and bit shifting operations. A microchip with an array of individually addressable electrodes was employed to enable random access of the memory cells using electric fields. Three segments on a DNA template molecule were used to encode three data bits. Rapid writing of data bits was enabled by electric field-induced hybridization of fluorescently labeled complementary probes and the data bits were read by fluorescence imaging. We demonstrated the rapid parallel writing and reading of 8 (2) combinations of 3-bit memory data and bit shifting operations by electric field-induced strand displacement. Our system may find potential applications in DNA-based memory and computations.
DNA已被用于存储数字信息或执行并行分子计算。在单个平台上结合基于DNA的存储和逻辑运算的能力相对未被探索。在此，我们展示了一种DNA三电平单元非易失性存储系统，其能够对存储器进行并行随机存取写入和移位操作。使用具有一系列可单独寻址电极的微芯片，通过电场实现对存储单元的随机访问。DNA模板分子上的三个片段用于编码三个数据位。通过电场诱导荧光标记的互补探针杂交实现数据位的快速写入，并通过荧光成像读取数据位。我们通过电场诱导的链置换展示了3位存储数据的8（2）种组合的快速并行写入和读取以及移位操作。我们的系统可能在基于DNA的存储和计算中找到潜在应用。
