Iwamoto Y, Murakami K, Danno M, Tsuchiya M, Masuzawa T, Shimizu T, Morita T, Yanagihara Y
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
J Pharmacobiodyn. 1990 May;13(5):316-20. doi: 10.1248/bpb1978.13.316.
Photobiological activities of pinacyanol chloride (PC), which is known as a non-intercalating dye, were investigated. Irradiation of PC-sensitized yeast cells in the dark brought about marked decrease of survival and induction of "petites" which are respiration-deficient mutants caused by partial loss of mitochondrial deoxyribonucleic acid. Nuclear mutation represented by reversion from Trp- to Trp+ was also induced by photodynamic action of PC. This fact suggested photoactive dyes are not necessarily intercalated for inducing mitochondrial and nuclear mutation. Singlet oxygen production was determined in the photoirradiated PC solution by electron spin resonance spectrometry. Photobiological effects of PC might be brought about mainly by a type II photodynamic mechanism.
研究了作为非嵌入染料的氯化藻红蛋白（PC）的光生物学活性。在黑暗中对PC致敏的酵母细胞进行辐照，导致存活率显著降低，并诱导产生“小菌落”，这些“小菌落”是由线粒体脱氧核糖核酸部分缺失引起的呼吸缺陷型突变体。由色氨酸缺陷型（Trp-）回复为色氨酸野生型（Trp+）所代表的核突变也可由PC的光动力作用诱导产生。这一事实表明，光活性染料不一定需要嵌入才能诱导线粒体和核突变。通过电子自旋共振光谱法测定了光辐照PC溶液中单线态氧的产生。PC的光生物学效应可能主要由II型光动力机制引起。
