Equine Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
Ticks Tick Borne Dis. 2011 Jun;2(2):111-5. doi: 10.1016/j.ttbdis.2011.01.002. Epub 2011 Apr 1.
Anaplasma phagocytophilum is the aetiological agent of equine granulocytic anaplasmosis (EGA). The aim of this survey was to assess the prevalence of anti-A. phagocytophilum antibodies in the horse population of the Czech Republic (CZ) and to investigate possible links between seropositivity and the geographic origin, age, and/or sex of the tested horses. Antibodies against A. phagocytophilum were screened using an indirect fluorescent antibody method (IFA). Serum samples from 96 healthy horses from 8 localities (7 within the CZ and one control upland/tick-free locality in Ukraine) were examined. While the controls tested negative, the seroprevalence in the Czech localities was estimated at 73%, which suggests that subclinical EGA is common among horses in the CZ. Significant differences between seropositivity rates in individual stables were demonstrated (explicable by the different environment and/or management styles of the horses stabled therein). Horses in stables from which previous manifest cases had been reported tended to have higher average titres. Mares displayed a somewhat higher seropositivity rate than did stallions; age had no demonstrable effect upon the serological status of the examined horses.
嗜吞噬细胞无形体是马粒细胞无形体病（EGA）的病原体。本研究旨在评估捷克共和国（CZ）马群中抗嗜吞噬细胞无形体抗体的流行情况，并调查血清阳性与测试马匹的地理来源、年龄和/或性别之间的可能联系。使用间接荧光抗体法（IFA）筛选针对 A. phagocytophilum 的抗体。检查了来自 8 个地点（CZ 内的 7 个和乌克兰的一个高地/无蜱控制地点）的 96 匹健康马的血清样本。虽然对照呈阴性，但在捷克地点的血清阳性率估计为 73%，这表明 CZ 中的马中存在亚临床 EGA 很常见。在各个马厩的血清阳性率之间显示出显著差异（可通过其中马匹的不同环境和/或管理方式来解释）。曾报告有明显病例的马厩中的马往往具有更高的平均效价。母马的血清阳性率略高于公马；年龄对检查马匹的血清学状态没有明显影响。
