Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Dev Biol. 2011 Sep 15;357(2):326-35. doi: 10.1016/j.ydbio.2011.07.001. Epub 2011 Jul 13.
NudC is a highly conserved protein necessary for cytoplasmic dynein-mediated nuclear migration in Aspergillus nidulans. NudC interacts genetically with Aspergillus NudF and physically with its mammalian orthologue Lis1, which is crucial for nuclear and neuronal migration during brain development. To test for related roles for NudC, we performed in utero electroporation into embryonic rat brain of cDNAs encoding shRNAs as well as wild-type and mutant forms of NudC. We show here that NudC, like Lis1, is required for neuronal migration during neocorticogenesis and we identify a specific role in apical nuclear migration in radial glial progenitor cells. These results identify a novel neuronal migration gene with a specific role in interkinetic nuclear migration, consistent with cytoplasmic dynein regulation.
NudC 是一种高度保守的蛋白质，对于曲霉菌中的细胞质动力蛋白介导的核迁移是必需的。NudC 在遗传上与曲霉菌 NudF 相互作用，在物理上与它的哺乳动物同源物 Lis1 相互作用，Lis1 对于大脑发育过程中的核和神经元迁移至关重要。为了测试 NudC 的相关作用，我们将编码 shRNA 以及野生型和突变型 NudC 的 cDNA 进行胚胎大鼠脑内的电穿孔。我们在这里表明，NudC 像 Lis1 一样，对于新皮质发生过程中的神经元迁移是必需的，并且我们确定了在放射状神经胶质祖细胞中的顶端核迁移中的特定作用。这些结果鉴定了一个新的神经元迁移基因，它在有丝分裂核迁移中具有特定作用，与细胞质动力蛋白的调节一致。
