从实验饲养的小鼠中分离和鉴定耐多药溶血葡萄球菌。

Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse.

机构信息

Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China.

出版信息

Asian Pac J Trop Med. 2011 Jun;4(6):421-5. doi: 10.1016/S1995-7645(11)60118-1.

DOI:10.1016/S1995-7645(11)60118-1

PMID:21771691

Abstract

OBJECTIVE

To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away from a hospital.

METHODS

Phenotype of the isolate was investigated by conventional microbiological methods, including Gram-staining, colony morphology, tests for haemolysis, catalase, coagulase, and antimicrobial susceptibility test. The mecA and 16S rRNA genes were amplified by the polymerase chain reaction (PCR) and sequenced. The base sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank database by phylogenetic analysis and multiple sequence alignment.

RESULTS

The isolate in this study was a gram positive, coagulase negative, and catalase positive coccus. The isolate was resistant to oxacillin, methicillin, penicillin, ampicillin, cefazolin, ciprofloxacin erythromycin, et al. PCR results indicated that the isolate was mecA gene positive and its 16S rRNA was 1 465 bp. Phylogenetic analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus, and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the GenBank.

CONCLUSIONS

16S rRNA gene sequencing is a suitable technique for non-specialist researchers. Laboratory animals are possible sources of lethal pathogens, and researchers must adapt protective measures when they manipulate animals.

摘要

目的

分析并鉴定一株远离医院的实验室饲养小鼠分离出的细菌菌株。

方法

采用常规微生物学方法（包括革兰氏染色、菌落形态、溶血试验、触酶试验、凝固酶试验和抗菌药物敏感性试验）对分离株进行表型分析。采用聚合酶链反应（PCR）扩增 mecA 和 16S rRNA 基因，并进行测序。通过系统发育分析和多序列比对，将 PCR 产物的碱基序列与 GenBank 数据库中已知的 16S rRNA 基因序列进行比较。

结果

本研究中的分离株为革兰氏阳性、凝固酶阴性、触酶阳性球菌。该分离株对苯唑西林、甲氧西林、青霉素、氨苄西林、头孢唑林、环丙沙星、红霉素等耐药。PCR 结果表明，该分离株 mecA 基因阳性，其 16S rRNA 为 1465bp。所得 16S rRNA 的系统发育分析表明，该分离株属于葡萄球菌属，与 GenBank 中已存入的相应 16S rRNA 相比，多序列比对显示该分离株为溶血性葡萄球菌，仅存在一个碱基差异。