海洋放线菌对多重耐药金黄色葡萄球菌的体外抗菌活性
In-vitro antimicrobial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus.
作者信息
Sathish Kumar S R, Kokati Venkata Bhaskara Rao
机构信息
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India.
出版信息
Asian Pac J Trop Biomed. 2012 Oct;2(10):787-92. doi: 10.1016/S2221-1691(12)60230-5.
OBJECTIVE
To investigate the antibacterial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus (MDRSA).
METHODS
Fifty one actinobacterial strains were isolated from salt pans soil, costal area in Kothapattanam, Ongole, Andhra Pradesh. Primary screening was done using cross-streak method against MDRSA. The bioactive compounds are extracted from efficient actinobacteria using solvent extraction. The antimicrobial activity of crude and solvent extracts was performed using Kirby-Bauer method. MIC for ethyl acetate extract was determined by modified agar well diffusion method. The potent actinobacteria are identified using Nonomura key, Shirling and Gottlieb 1966 with Bergey's manual of determinative bacteriology.
RESULTS
Among the fifty one isolates screened for antibacterial activity, SRB25 were found efficient against MDRSA. The ethyl acetate extracts showed high inhibition against test organism. MIC test was performed with the ethyl acetate extract against MDRSA and found to be 1 000 µg/mL. The isolated actinobacteria are identified as Streptomyces sp with the help of Nonomura key.
CONCLUSIONS
The current investigation reveals that the marine actinobacteria from salt pan environment can be able to produce new drug molecules against drug resistant microorganisms.
目的
研究海洋放线菌对多重耐药金黄色葡萄球菌（MDRSA）的抗菌活性。
方法
从印度安得拉邦翁戈勒科塔帕塔纳姆沿海地区的盐田土壤中分离出51株放线菌菌株。采用交叉划线法对MDRSA进行初步筛选。使用溶剂萃取法从高效放线菌中提取生物活性化合物。采用 Kirby-Bauer 法检测粗提物和溶剂提取物的抗菌活性。采用改良琼脂孔扩散法测定乙酸乙酯提取物的最低抑菌浓度（MIC）。利用野村检索表、1966年 Shirling 和 Gottlieb 方法以及《伯杰氏鉴定细菌学手册》对强效放线菌进行鉴定。
结果
在筛选的51株具有抗菌活性的菌株中，发现SRB25对MDRSA有效。乙酸乙酯提取物对受试菌表现出高度抑制作用。对乙酸乙酯提取物进行MDRSA的MIC测试，结果为1000μg/mL。借助野村检索表将分离出的放线菌鉴定为链霉菌属。
结论
当前研究表明，来自盐田环境的海洋放线菌能够产生针对耐药微生物的新药物分子。
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