来自人类和伴侣动物感染的表皮葡萄球菌和溶血葡萄球菌分离株的比较基因型、葡萄球菌盒式染色体mec（SCCmec）基因及抗菌药物耐药性
Comparative Genotypes, Staphylococcal Cassette Chromosome mec (SCCmec) Genes and Antimicrobial Resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus Isolates from Infections in Humans and Companion Animals.
作者信息
McManus Brenda A, Coleman David C, Deasy Emily C, Brennan Gráinne I, O' Connell Brian, Monecke Stefan, Ehricht Ralf, Leggett Bernadette, Leonard Nola, Shore Anna C
机构信息
Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.
Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland; National MRSA Reference Laboratory, St. James's Hospital, James's St., Dublin 8, Ireland.
出版信息
PLoS One. 2015 Sep 17;10(9):e0138079. doi: 10.1371/journal.pone.0138079. eCollection 2015.
This study compares the characteristics of Staphylococcus epidermidis (SE) and Staphylococcus haemolyticus (SH) isolates from epidemiologically unrelated infections in humans (Hu) (28 SE-Hu; 8 SH-Hu) and companion animals (CpA) (12 SE-CpA; 13 SH-CpA). All isolates underwent antimicrobial susceptibility testing, multilocus sequence typing and DNA microarray profiling to detect antimicrobial resistance and SCCmec-associated genes. All methicillin-resistant (MR) isolates (33/40 SE, 20/21 SH) underwent dru and mecA allele typing. Isolates were predominantly assigned to sequence types (STs) within a single clonal complex (CC2, SE, 84.8%; CC1, SH, 95.2%). SCCmec IV predominated among MRSE with ST2-MRSE-IVc common to both Hu (40.9%) and CpA (54.5%). Identical mecA alleles and nontypeable dru types (dts) were identified in one ST2-MRSE-IVc Hu and CpA isolate, however, all mecA alleles and 2/4 dts detected among 18 ST2-MRSE-IVc isolates were closely related, sharing >96.5% DNA sequence homology. Although only one ST-SCCmec type combination (ST1 with a non-typeable [NT] SCCmec NT9 [class C mec and ccrB4]) was common to four MRSH-Hu and one MRSH-CpA, all MRSH isolates were closely related based on similar STs, SCCmec genes (V/VT or components thereof), mecA alleles and dts. Overall, 39.6% of MR isolates harbored NT SCCmec elements, and ACME was more common amongst MRSE and CpA isolates. Multidrug resistance (MDR) was detected among 96.7% of isolates but they differed in the prevalence of specific macrolide, aminoglycoside and trimethoprim resistance genes amongst SE and SH isolates. Ciprofloxacin, rifampicin, chloramphenicol [fexA, cat-pC221], tetracycline [tet(K)], aminoglycosides [aadD, aphA3] and fusidic acid [fusB] resistance was significantly more common amongst CpA isolates. SE and SH isolates causing infections in Hu and CpA hosts belong predominantly to STs within a single lineage, harboring similar but variable SCCmec genes, mecA alleles and dts. Host and staphylococcal species-specific characteristics were identified in relation to antimicrobial resistance genes and phenotypes, SCCmec and ACME.
本研究比较了从人类（Hu）（28株表皮葡萄球菌-Hu；8株溶血葡萄球菌-Hu）和伴侣动物（CpA）（12株表皮葡萄球菌-CpA；13株溶血葡萄球菌-CpA）的流行病学无关感染中分离出的表皮葡萄球菌（SE）和溶血葡萄球菌（SH）的特征。所有分离株均进行了抗菌药物敏感性测试、多位点序列分型和DNA微阵列分析，以检测抗菌药物耐药性和SCCmec相关基因。所有耐甲氧西林（MR）分离株（33/40 SE，20/21 SH）进行了dru和mecA等位基因分型。分离株主要被归入单个克隆复合体（CC2，SE，84.8%；CC1，SH，95.2%）内的序列类型（STs）。SCCmec IV在MRSE中占主导地位，Hu（40.9%）和CpA（54.5%）中常见的是ST2-MRSE-IVc。在一株ST2-MRSE-IVc Hu和CpA分离株中鉴定出相同的mecA等位基因和不可分型的dru类型（dts），然而，在18株ST2-MRSE-IVc分离株中检测到的所有mecA等位基因和2/4 dts密切相关，DNA序列同源性>96.5%。虽然只有一种ST-SCCmec类型组合（ST1与不可分型[NT] SCCmec NT9 [C类mec和ccrB4]）在四株MRSH-Hu和一株MRSH-CpA中常见，但所有MRSH分离株基于相似的STs、SCCmec基因（V/VT或其组成部分）、mecA等位基因和dts密切相关。总体而言，39.6%的MR分离株携带NT SCCmec元件，ACME在MRSE和CpA分离株中更常见。在96.7%的分离株中检测到多重耐药（MDR），但SE和SH分离株中特定大环内酯类抗生素、氨基糖苷类抗生素和甲氧苄啶耐药基因的流行率有所不同。环丙沙星、利福平、氯霉素[fexA，cat-pC221]、四环素[tet(K)]、氨基糖苷类抗生素[aadD，aphA3]和夫西地酸[fusB]耐药在CpA分离株中明显更常见。在Hu和CpA宿主中引起感染的SE和SH分离株主要属于单个谱系内的STs，携带相似但可变的SCCmec基因、mecA等位基因和dts。在抗菌药物耐药基因和表型、SCCmec和ACME方面鉴定出宿主和葡萄球菌物种特异性特征。
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