Shriners Hospitals for Children Northern California and Department of Surgery, University of California, Davis, 2425 Stockton Boulevard, Sacramento, CA 95817, USA.
Mediators Inflamm. 2011;2011:509604. doi: 10.1155/2011/509604. Epub 2011 Jun 5.
Envelope (env) proteins of certain endogenous retroviruses (ERVs) participate in various pathophysiological processes. In this study, we characterized pathophysiologic properties of two murine leukemia virus-type ERV (MuLV-ERV) env genes cloned from the ovary of C57BL/6J mice. The two env genes (named ENV(OV1) and ENV(OV2)), with 1,926 bp coding region, originated from two MuLV-ERV loci on chromosomes 8 and 18, respectively. ENV(OV1) and ENV(OV2) were ~75 kDa and predominantly expressed on the cell membrane. They were capable of producing pseudotype murine leukemia virus virions. Tropism trait and infectivity of ENV(OV2) were similar to the polytropic env; however, ENV(OV1) had very low level of infectivity. Overexpression of ENV(OV2), but not ENV(OV1), exerted cytotoxic effects and induced expression of COX-2, IL-1β, IL-6, and iNOS. These findings suggest that the ENV(OV1) and ENV(OV2) are capable of serving as an env protein for virion assembly, and they exert differential cytotoxicity and modulation of inflammatory mediators.
某些内源性逆转录病毒（ERV）的包膜（env）蛋白参与各种病理生理过程。在这项研究中，我们对从 C57BL/6J 小鼠卵巢中克隆的两种小鼠白血病病毒型 ERV（MuLV-ERV）env 基因的病理生理特性进行了表征。这两个 env 基因（命名为 ENV(OV1)和 ENV(OV2)），编码区为 1926bp，分别来自染色体 8 和 18 上的两个 MuLV-ERV 基因座。ENV(OV1)和 ENV(OV2)的大小约为 75kDa，主要表达在细胞膜上。它们能够产生假型鼠白血病病毒病毒颗粒。ENV(OV2)的嗜性和感染性与多嗜性 env 相似；然而，ENV(OV1)的感染性非常低。ENV(OV2)的过表达而非 ENV(OV1)的过表达会产生细胞毒性作用，并诱导 COX-2、IL-1β、IL-6 和 iNOS 的表达。这些发现表明，ENV(OV1)和 ENV(OV2)能够作为病毒粒子组装的 env 蛋白，它们发挥不同的细胞毒性作用和调节炎症介质。
