Lévican Jorge, Torres Mauricio, Gaggero Nicolás, Corvalán Rosa, Gaggero Aldo
Programa de Virología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Rev Med Chil. 2011 Feb;139(2):143-9. Epub 2011 Jul 11.
Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions.
To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases.
A total of 477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR.
Parvovirus B19 was detected in four samples, rendering a frequency of 1:119. The viral loads ranged from less than 2000 to 5626 x 10(5) genome equivalents/ml.
Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago.
细小病毒B19（B19）与人类多种疾病相关，其严重程度取决于宿主的免疫和血液学状态。它主要通过呼吸道传播，但也可通过输血传播。
确定智利圣地亚哥三家医院血库的志愿献血者群体中B19 DNA携带者频率，并确定DNA阳性病例中的病毒载量。
共分析了477份血清样本。通过针对病毒非结构区域（NS1）的巢式聚合酶链反应（PCR）对B19 DNA进行筛查。通过NS1实时PCR对阳性病例中的病毒载量进行定量。
在4份样本中检测到细小病毒B19，频率为1:119。病毒载量范围为每毫升少于2000至5626×10⁵基因组当量。
在圣地亚哥三家医院的477名血库献血者中，有4人感染了细小病毒B19。
