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对选定宿主中的微孢子虫进行分子鉴定和基因分型。

Molecular identification and genotyping of Microsporidia in selected hosts.

机构信息

University of Veterinary Medicine and Pharmacy In Košice, Komenského 73, 041 81 Košice, Slovakia.

出版信息

Parasitol Res. 2012 Feb;110(2):689-93. doi: 10.1007/s00436-011-2543-9. Epub 2011 Jul 20.

DOI:10.1007/s00436-011-2543-9
PMID:21773772
Abstract

The work is described by microscopic analysis, the serological analysis (IFAT) and the molecular analysis of isolates from clinical samples (blood, faeces and urine) from ten domestic rabbits (Oryctolagus cuniculus), breed Maličký, four New Zealand domestic rabbits, 11 sows of breed Slo0076akian Improved White and 15 clinically healthy laboratory BALB/c mice. The aim of the study was to validate the suitability of species-unspecific primer pairs 530F and 580R for genotype determination of the Microsporidia strain and species-specific primer pairs ECUNF and ECUNR, SINTF and SINTR and EBIER1 and EBIEF1 for the determination of E ncephalitozoon cuniculi, Encephalitozoon intestinalis and Enterocytozoon bieneusi species for diagnostic purposes. Sequences of animals were compared with those from the GenBank database. In rabbits, two murine genotypes II and four canine genotypes III were identified. Genotype II was identified in mice. The Encephalitozoon intestinalis identified in the sample from swine showed no genetic heterogeneity.

摘要

该工作通过微观分析、血清学分析（IFA）和从 10 只家兔（Oryctolagus cuniculus）、4 只新西兰家兔、11 头 Slo0076akian 改良白猪和 15 只临床健康的实验室 BALB/c 小鼠的临床样本（血液、粪便和尿液）中分离株的分子分析进行了描述。该研究的目的是验证非种特异性引物对 530F 和 580R 用于确定 Microsporidia 菌株基因型的适用性，以及物种特异性引物对 ECUNF 和 ECUNR、SINTF 和 SINTR 和 EBIER1 和 EBIEF1 用于确定 E ncephalitozoon cuniculi、Encephalitozoon intestinalis 和 Enterocytozoon bieneusi 物种的诊断目的。对动物序列与 GenBank 数据库中的序列进行了比较。在家兔中，鉴定出两种鼠基因型 II 和四种犬基因型 III。在小鼠中鉴定出基因型 II。从猪样本中鉴定出的肠脑炎原虫没有遗传异质性。

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