从猪脑中纯化微管蛋白。

Purification of tubulin from porcine brain.

作者信息

Gell Christopher, Friel Claire T, Borgonovo Barbara, Drechsel David N, Hyman Anthony A, Howard Jonathon

机构信息

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

出版信息

Methods Mol Biol. 2011;777:15-28. doi: 10.1007/978-1-61779-252-6_2.

DOI:10.1007/978-1-61779-252-6_2

PMID:21773918

Abstract

Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.

摘要

微管是由异二聚体蛋白αβ-微管蛋白聚合而成，赋予细胞形状，是囊泡运输和染色体分离的轨道。研究微管功能及其受微管相关蛋白调控的体外实验需要有纯化的αβ-微管蛋白。在本章中，我们描述了从猪脑中纯化异二聚体αβ-微管蛋白的过程。