高钙溶液可预防氨基糖苷类抗生素对大鼠海马切片中枢突触传递的抑制作用。

High calcium solutions prevent the depressant effects of aminoglycoside antibiotics on central synaptic transmission in rat hippocampal slices.

Sagratella S, Proietti M L, Frank C, Scotti de Carolis A

Pharmacology Department, Istituto Superiore di Sanita, Roma, Italy.

Gen Pharmacol. 1990;21(5):635-8. doi: 10.1016/0306-3623(90)91010-o.

Abstract

The aminoglycoside antibiotics neomycin and streptomycin were tested on the synaptic activity of the CA1 pyramidal neuron-Schaffer collateral interconnections in rat hippocampal slices. 2. Neomycin (0.5 mM) decreased the magnitude and shifted to the right the stimulus-response curve of the somatic and dendritic excitatory postsynaptic potential (EPSP) and the somatic population spike (PS). 3. Streptomycin (1 mM) decreased the magnitude and shifted to the right the stimulus-response curve of the somatic and dendritic EPSP and the somatic PS. 4. High (+2 mM) calcium solutions were able to prevent the effects induced by the antibiotics and to shift to the left the stimulus-response curve of the 0.5 mM neomycin and 1 mM streptomycin treated hippocampal slices. 5. The data demonstrate an effect of aminoglycoside antibiotics on a central mammalian hippocampal synapses, that may depend on an interference of the drugs on calcium conductances.

摘要

对氨基糖苷类抗生素新霉素和链霉素进行了测试，观察它们对大鼠海马切片中CA1锥体神经元 - 谢弗侧支突触连接的突触活性的影响。2. 新霉素（0.5 mM）降低了幅度，并使躯体和树突兴奋性突触后电位（EPSP）以及躯体群体峰电位（PS）的刺激 - 反应曲线向右移动。3. 链霉素（1 mM）降低了幅度，并使躯体和树突EPSP以及躯体PS的刺激 - 反应曲线向右移动。4. 高钙（+2 mM）溶液能够预防抗生素诱导的效应，并使经0.5 mM新霉素和1 mM链霉素处理的海马切片的刺激 - 反应曲线向左移动。5. 数据表明氨基糖苷类抗生素对中枢哺乳动物海马突触有影响，这可能取决于药物对钙电导的干扰。