百日咳毒素可预防新霉素诱导的大鼠海马切片中钙依赖性电生理效应。

Pertussis toxin prevents neomycin-induced calcium-dependent electrophysiological effects in rat hippocampal slices.

Frank C, Longo R, Sagratella S

Laboratorio di Farmacologia, Istituto Superiore di Sanitá, Roma, Italy.

Gen Pharmacol. 1994 Sep;25(5):957-61. doi: 10.1016/0306-3623(94)90103-1.

Abstract

The influence of pertussis toxin has been studied on the effects of neomycin on CA1 field potentials in rat hippocampal slices in order to determine a role played by G protein in the modulation of synaptic transmission by the drug. 2. Neomycin (500 microM), within 30 min significantly (P < 0.01) decreased the magnitude of the somatic CA1 excitatory postsynaptic potentials (EPSP) and population spike (PS) in control hippocampal slices. 3. Neomycin (500 microM), within 30 min failed to significantly affect the magnitude of the somatic CA1 EPSP and PS in slices obtained from animals treated intracerebroventricularly (ICV) with 1-2 micrograms of pertussis toxin 3 days before. 4. The results demonstrated that pertussis toxin prevents some electrophysiological effects of neomycin, suggesting a role of G protein in the modulation of the aminoglycoside antibiotic on central synaptic transmission.

摘要

为了确定G蛋白在该药物对突触传递的调节中所起的作用，研究了百日咳毒素对新霉素对大鼠海马切片CA1区场电位影响的作用。2. 在对照海马切片中，新霉素（500微摩尔）在30分钟内显著（P<0.01）降低了体细胞CA1兴奋性突触后电位（EPSP）和群体峰电位（PS）的幅度。3. 对于3天前经脑室内（ICV）注射1-2微克百日咳毒素处理的动物所获得的切片，新霉素（500微摩尔）在30分钟内未能显著影响体细胞CA1 EPSP和PS的幅度。4. 结果表明，百日咳毒素可阻止新霉素的某些电生理效应，提示G蛋白在氨基糖苷类抗生素对中枢突触传递的调节中发挥作用。