Department of Chemistry, Fudan University, Shanghai, 200433, PR China.
Inorg Chem. 2011 Aug 15;50(16):7412-20. doi: 10.1021/ic102082k. Epub 2011 Jul 20.
In this work, a neutral iridium(III) complex [Ir(bt)(2)(acac)] (Hbt = 2-phenylbenzothiazole; Hacac = acetylacetone) has been realized as a Hg(II)-selective sensor through UV-vis absorption, phosphorescence emission, and electrochemical measurements and was further developed as a phosphorescent agent for monitoring intracellular Hg(II). Upon addition of Hg(II) to a solution of [Ir(bt)(2)(acac)], a noticeable spectral blue shift in both absorption and phosphorescent emission bands was measured. (1)H NMR spectroscopic titration experiments indicated that coordination of Hg(II) to the complex induces fast decomposition of [Ir(bt)(2)(acac)] to form a new complex, which is responsible for the significant variations in optical and electrochemical signals. Importantly, cell imaging experiments have shown that [Ir(bt)(2)(acac)] is membrane permeable and can be used to monitor the changes in Hg(II) levels within cells in a ratiometric phosphorescence mode.
在这项工作中，通过紫外-可见吸收、磷光发射和电化学测量，实现了中性铱(III)配合物[Ir(bt)(2)(acac)]（Hbt = 2-苯基苯并噻唑；Hacac = 乙酰丙酮）作为 Hg(II)选择性传感器，并进一步开发为用于监测细胞内 Hg(II)的磷光探针。向[Ir(bt)(2)(acac)]溶液中加入 Hg(II)后，测量到吸收和磷光发射带均有明显的光谱蓝移。(1)H NMR 光谱滴定实验表明，Hg(II)与配合物的配位诱导[Ir(bt)(2)(acac)]快速分解，形成新的配合物，这是光学和电化学信号显著变化的原因。重要的是，细胞成像实验表明，[Ir(bt)(2)(acac)]具有膜透过性，可用于以比率磷光模式监测细胞内 Hg(II)水平的变化。
