Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, Taiwan.
Tissue Eng Part A. 2011 Nov;17(21-22):2593-602. doi: 10.1089/ten.tea.2011.0080. Epub 2011 Jul 20.
The effect of visible light irradiation on the expression of pluripotent genes (Oct-4, Sox2, and Nanog) in amniotic fluid-derived stem cells (AFSCs) and on the osteogenic differentiation ability of AFSCs was investigated using light-emitting diodes (LEDs) at 0-2 mW/cm(2) in various wavelengths: [blue (470 nm), green (525 nm), yellow (600 nm), and red (630 nm)]. Pluripotent gene expression in AFSCs was up-regulated by visible light irradiation from a LED for more than 6 h. Green light irradiation of AFSCs up-regulated the expression of pluripotent genes more significantly than irradiation with other light. The osteogenic differentiation of AFSCs was facilitated by green and blue light irradiation. Facilitated differentiation into osteogenic cells by visible light irradiation was not mediated by reactive oxygen species (ROS); alkaline phosphatase activity (a marker of early osteogenic differentiation) and gene expression of osteopontin (a marker of late osteogenic differentiation) did not change significantly between AFSCs in differentiation medium with or without a ROS scavenger (vitamin C). The mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathway, as well as other unknown signaling pathways, may be responsible for the activation of signaling pathways that facilitate the differentiation of AFSCs into osteogenic cells on light irradiation.
研究了发光二极管（LED）在 0-2 mW/cm(2) 不同波长（[蓝色（470nm）、绿色（525nm）、黄色（600nm）和红色（630nm）]）下对羊膜间充质干细胞（AFSCs）中多能基因（Oct-4、Sox2 和 Nanog）表达的影响及其对 AFSCs 成骨分化能力的影响。AFSCs 经 LED 可见光照射超过 6 小时后，多能基因表达上调。绿光对 AFSCs 中多能基因的上调作用比其他光更显著。绿光和蓝光照射促进了 AFSCs 的成骨分化。可见光照射促进成骨细胞分化并非由活性氧（ROS）介导；分化培养基中有无 ROS 清除剂（维生素 C），碱性磷酸酶活性（成骨早期分化的标志物）和成骨蛋白（晚期成骨分化的标志物）的基因表达均无明显变化。丝裂原活化蛋白激酶/细胞外信号调节蛋白激酶通路以及其他未知信号通路可能负责激活信号通路，促进 AFSCs 向成骨细胞分化。
