Higuchi Akon, Wang Ching-Tang, Ling Qing-Dong, Lee Henry Hsin-chung, Kumar S Suresh, Chang Yung, Alarfaj Abdullah A, Munusamy Murugan A, Hsu Shih-Tien, Wu Gwo-Jang, Umezawa Akihiko
1] Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan, Taiwan [2] Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia [3] Department of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan [4] Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan.
Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan, Taiwan.
Sci Rep. 2015 May 13;5:10217. doi: 10.1038/srep10217.
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method.
人脂肪来源干细胞（hADSCs）具有异质性特征，表明其具有多种基因型和分化能力。根据所使用的纯化方法，分离出的hADSCs可能具有不同的纯度水平和不同的特性。我们开发了一种混合膜迁移方法，可从脂肪组织溶液中以极高的纯度和多能性纯化hADSCs。将原代脂肪组织溶液通过孔径为8至25μm的多孔膜渗透，然后将这些膜在细胞培养基中孵育15 - 18天。从膜迁移出来的hADSCs中，间充质干细胞标志物阳性细胞的比例极高（例如，>98%），并且与使用传统培养方法分离的细胞相比，一些多能性基因（Oct4、Sox2、Klf4和Nanog）的表达几乎高出一个数量级。
