Center of Biological Diagnosis and Therapy, No, 261 Hospital of PLA, Beijing 100094, China.
Nanoscale Res Lett. 2011 Jul 20;6(1):461. doi: 10.1186/1556-276X-6-461.
The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.
The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.
The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.
This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.
纳米材料研究领域的最新进展极大地推动了传染病诊断学的发展。许多纳米材料已经被开发出来并应用于实验室中的分子诊断。目前，人乳头瘤病毒（HPV）的诊断测试完全依赖于分子测试。在此，我们报告了一种快速简便的量子点（QD）和超顺磁性纳米粒子杂交检测方法，用于检测 HPV16 感染，该方法结合了超顺磁性纳米粒子和 QD 的优点，与传统的杂交检测方法完全不同，因为反应在均相溶液中进行，检测总时间不超过 1 小时。
探针用超顺磁性纳米粒子和 QD 标记。用这些探针对 60 例宫颈拭子样本进行杂交检测，并与 HPV 特异性聚合酶链反应（PCR）方法进行比较。
统计分析表明，这两种方法没有显著差异。此外，这种方法比 HPV 特异性 PCR 方法更快、更容易。
本研究成功验证了我们的杂交检测方法的临床性能。在检测时间和操作简便性方面的优势使该方法在临床应用中具有巨大的潜力，特别是在大规模的流行病学筛查中。
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