Department of Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.
Fertil Steril. 2011 Sep;96(3):624-8. doi: 10.1016/j.fertnstert.2011.06.060. Epub 2011 Jul 20.
To create a pool of frozen donated human oocytes and find the optimal stage for slow controlled-rate freezing of human in vitro matured oocytes.
Oocytes at different developmental stages of maturation (germinal vesicle, metaphase I, or metaphase II) and oocytes that failed to fertilize after IVF or intracytoplasmic sperm injection (ICSI) were frozen using a slow controlled-rate freezing protocol. Frozen/thawed oocytes were artificially activated to verify activation potential and compared with oocytes that were not frozen.
University hospital-based fertility center.
PATIENT(S): Stimulated patients undergoing IVF/ICSI treatment donated oocytes left over during their infertility treatment.
INTERVENTION(S): Human oocytes were frozen at different stages of maturation. Fresh and frozen/thawed oocytes were activated by electrical pulses followed by incubation in 6-dimethylaminopurine.
MAIN OUTCOME MEASURE(S): Survival rate, maturation rate and kinetics, and activation potential.
RESULT(S): Human oocytes at all developmental stages have high survival rates after slow controlled-rate freezing. Frozen/thawed germinal vesicle oocytes showed decreased and delayed maturation after thawing. Activation potential was not affected.
CONCLUSION(S): A pool of donated human oocytes could be established using slow controlled-rate freezing. Immature oocytes should be frozen after in vitro maturation.
建立冷冻捐献人类卵母细胞库，并寻找体外成熟卵母细胞慢控冷冻的最佳阶段。
使用慢控冷冻方案对处于不同成熟阶段（生发泡、MⅠ或 MⅡ）的卵母细胞以及体外受精或胞浆内单精子注射后未受精的卵母细胞进行冷冻。冷冻/解冻卵母细胞被人工激活以验证激活潜能，并与未冷冻的卵母细胞进行比较。
以大学医院为基础的生育中心。
接受 IVF/ICSI 治疗的刺激患者捐献了在其不孕治疗过程中剩余的卵母细胞。
将人类卵母细胞在不同的成熟阶段进行冷冻。新鲜和冷冻/解冻卵母细胞通过电脉冲激活，然后在 6-二甲基氨基嘌呤中孵育。
存活率、成熟率和动力学以及激活潜能。
慢控冷冻后，所有发育阶段的人类卵母细胞均具有较高的存活率。解冻后的冷冻生发泡卵母细胞显示出成熟率降低和成熟延迟。激活潜能不受影响。
可以使用慢控冷冻建立捐献人类卵母细胞库。体外成熟后应冷冻不成熟卵母细胞。
