Research Laboratory on Human Reproduction, Medicine Faculty, Université Libre de Bruxelles, Belgium, Campus Erasme, Brussels, Belgium.
J Assist Reprod Genet. 2012 Jun;29(6):507-12. doi: 10.1007/s10815-012-9751-9. Epub 2012 Apr 4.
This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification.
A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2).
Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p < 0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p < 0.05).
IVM procedure is more efficient when it is performed before oocyte vitrification.
本研究旨在确定人类未成熟卵母细胞的体外成熟（IVM）应在玻璃化之前还是之后进行。
共随机将 184 个未成熟卵母细胞分为两组：100 个在 IVM 后 24-48 小时的 MII 期进行玻璃化（第 1 组），84 个在 GV 或 MI 期立即进行玻璃化，并在体外成熟后解冻（第 2 组）。
两组解冻后的存活率相似（86.9%对 84.5%）。然而，每采集一个卵母细胞的卵母细胞成熟率在玻璃化前成熟的卵母细胞（第 1 组，46%）明显高于玻璃化前 IVM 的卵母细胞（第 2 组，23.8%）（p < 0.01）。因此，第 1 组（40%）每采集一个卵母细胞的 MII 卵母细胞受精数明显高于第 2 组（23.8%）（p < 0.05）。
在卵母细胞玻璃化之前进行 IVM 程序效率更高。
