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体外成熟前后玻璃化冷冻的人卵母细胞的超微结构特征

Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation.

作者信息

Segovia Yolanda, Victory Noemí, Peinado Irene, García-Valverde Laura M, García Magdalena, Aizpurua Jon, Monzó Ana, Gómez-Torres María José

机构信息

Departamento de Biotecnología, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain.

Unidad de Reproducción Humana, Hospital La Fe, Valencia, Spain.

出版信息

J Reprod Dev. 2017 Aug 19;63(4):377-382. doi: 10.1262/jrd.2017-009. Epub 2017 Apr 30.

DOI:10.1262/jrd.2017-009
PMID:28458301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5593089/
Abstract

The development of an effective program that combines in vitro maturation (IVM) and cryopreservation for immature oocytes would represent a novel advance for in vitro fertilization (IVF), especially as a means to preserve the fertility of women in unique situations. The aim of this study was to analyze the ultrastructural characteristics of human oocytes, obtained after controlled ovarian stimulation, to determine whether IVM is best performed before or after vitrification. To this end, we analyzed the following features in a total of 22 MII oocytes: size, zona pellucida and perivitelline space, mitochondria number, M-SER (mitochondria-smooth endoplasmic reticulum) aggregates and M-V (mitochondria-vesicle) complexes, the number of cortical granules and microvilli, and the presence of vacuolization using transmission electron microscopy (TEM). Each oocyte presented a rounded shape, with an intact oolemma, and was surrounded by a continuous zona pellucida and perivitelline space. Statistical analysis comparing oocytes vitrified before or after IVM indicated that there were no significant differences between examined characteristics.

摘要

开发一种将未成熟卵母细胞的体外成熟（IVM）与冷冻保存相结合的有效方案，将代表体外受精（IVF）领域的一项新进展，尤其是作为在特殊情况下保存女性生育能力的一种手段。本研究的目的是分析在控制性卵巢刺激后获得的人类卵母细胞的超微结构特征，以确定IVM在玻璃化之前还是之后进行效果最佳。为此，我们总共分析了22个MII期卵母细胞的以下特征：大小、透明带和卵周间隙、线粒体数量、线粒体-滑面内质网（M-SER）聚集体和线粒体-囊泡（M-V）复合体、皮质颗粒和微绒毛的数量，以及使用透射电子显微镜（TEM）观察到的空泡化情况。每个卵母细胞呈圆形，卵膜完整，周围有连续的透明带和卵周间隙。比较IVM前后玻璃化的卵母细胞的统计分析表明，所检查的特征之间没有显著差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/4371cd30883a/jrd-63-377-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/e6589158397f/jrd-63-377-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/5f8dc7c7b6bb/jrd-63-377-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/939c9a427cbe/jrd-63-377-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/4371cd30883a/jrd-63-377-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/e6589158397f/jrd-63-377-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/5f8dc7c7b6bb/jrd-63-377-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/939c9a427cbe/jrd-63-377-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/5593089/4371cd30883a/jrd-63-377-g004.jpg

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