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人工重组可能会影响新城疫病毒的进化分析。

Artificial recombination may influence the evolutionary analysis of Newcastle disease virus.

机构信息

College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

出版信息

J Virol. 2011 Oct;85(19):10409-14. doi: 10.1128/JVI.00544-11. Epub 2011 Jul 20.

Abstract

The recombination rate in Newcastle disease virus (NDV) was as high as 10% in RDP analysis with full-length NDV genome sequences available in GenBank. We found that two NDV strains, China/Guangxi09/2003 and NDV/03/018, previously reported as recombinants, failed to show any evidence of recombination upon complete genome resequencing. Furthermore, we were able to reproduce artificial recombination by amplification of the M gene in a mixed sample of strains LaSota and ZJ1. It appears that the recombination of NDV is not as common as has been reported. NDV sequences in GenBank should be analyzed with caution during bioinformatic analyses for natural recombination events.

摘要

在 RDP 分析中,利用 GenBank 中提供的全长新城疫病毒(NDV)基因组序列,NDV 的重组率高达 10%。我们发现,之前报道为重组体的两株 NDV 株,中国/广西 09/2003 和 NDV/03/018,在进行全基因组重测序后并未显示出任何重组的证据。此外,我们还能够通过扩增混合样本中的 LaSota 和 ZJ1 株的 M 基因来重现人工重组。看来,NDV 的重组并不像之前报道的那样常见。在进行自然重组事件的生物信息学分析时,应谨慎分析 GenBank 中的 NDV 序列。

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