Huang L, Li Z Y, Xu S Y, Chen M Z
Department of Physiology, Jiangxi Medical College, Nanchang.
Sheng Li Xue Bao. 1990 Oct;42(5):469-75.
The effects of thyroxine (L-T4) on the IgM production of splenocytes in mice induced by sheep red blood cell (SRBC) were investigated by means of spectrophotometry of quantitative hemolysis (QHS). The maximum binding site number (Bmax) and affinity (Kd) of beta-adrenergic receptors (beta-Adr. R.) on splenic lymphocytes of mice under the different thyroid functional status during immune response were determined by radio-ligand combination method. The IgM production and Bmax of beta- Adr. R. of splenic lymphocytes were increased in "hyperthyroid mice" and decreased in "hypothyroid mice" as compared with the control (P less than 0.01) during the 3rd to 5th day after immunization with SRBC, but affinity of beta-Adr. R. was not changed (P greater than 0.05). It is suggested that L-T4 could increase the IgM production in lymphocytes induced by SRBC in mice. The underlying mechanism seems to involve an increasing Bmax of beta-Adr. R. on the immune cell, thus enhancing its sensitivity to catecholamine.
采用定量溶血分光光度法（QHS）研究甲状腺素（L-T4）对绵羊红细胞（SRBC）诱导的小鼠脾细胞IgM产生的影响。采用放射性配体结合法测定免疫应答期间不同甲状腺功能状态下小鼠脾淋巴细胞β-肾上腺素能受体（β-Adr.R.）的最大结合位点数（Bmax）和亲和力（Kd）。与对照组相比，“甲状腺功能亢进小鼠”脾脏淋巴细胞的IgM产生量和β-Adr.R.的Bmax在SRBC免疫后第3至5天增加，“甲状腺功能减退小鼠”则降低（P<0.01），但β-Adr.R.的亲和力未改变（P>0.05）。提示L-T4可增加小鼠SRBC诱导的淋巴细胞中IgM的产生。其潜在机制似乎涉及免疫细胞上β-Adr.R.的Bmax增加，从而增强其对儿茶酚胺的敏感性。
