Fu Chi-Ling, Apelo Charity A, Torres Baldemar, Thai Kim H, Hsieh Michael H
Department of Urology, Stanford University School of Medicin, USA.
J Vis Exp. 2011 Jul 12(53):e2523. doi: 10.3791/2523.
Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. Before starting injections, the surgical area must be cleaned with soap and water and antiseptic solution. Surgical equipment must be sterilized before use and between each animal. Each mouse is placed under inhaled isoflurane anesthesia (2-5% for induction, 1-3% for maintenance) and its bladder exposed by making a midline abdominal incision with scissors. If the bladder is full, it is partially decompressed by gentle squeezing between two fingers. The cell suspension of interest is intramurally injected into the wall of the bladder dome using a 29 or 30 gauge needle and 1 cc or smaller syringe. The wound is then closed using wound clips and the mouse allowed to recover on a warming pad. Bladder wall injection is a delicate microsurgical technique that can be mastered with practice.
小鼠膀胱壁注射是原位研究膀胱现象的一种有用技术，包括干细胞、平滑肌和癌症生物学。在开始注射之前，手术区域必须用肥皂、水和消毒液清洗。手术设备在使用前以及对每只动物操作之间都必须进行消毒。每只小鼠置于吸入式异氟烷麻醉下（诱导时2 - 5%，维持时1 - 3%），用剪刀作腹部中线切口暴露膀胱。如果膀胱充盈，用两指轻轻挤压使其部分减压。使用29号或30号针头和1毫升或更小的注射器将感兴趣的细胞悬液壁内注射到膀胱顶部壁内。然后用伤口夹闭合伤口，让小鼠在保温垫上恢复。膀胱壁注射是一种精细的显微外科技术，通过练习可以掌握。
