Dinney C P, Fishbeck R, Singh R K, Eve B, Pathak S, Brown N, Xie B, Fan D, Bucana C D, Fidler I J
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
J Urol. 1995 Oct;154(4):1532-8.
These studies were designed to develop an orthotopic model for human bladder cancer and to isolate variant metastatic cell lines.
The human bladder cancer cell line 253J was implanted into the muscular wall of the bladder of athymic nude mice. By in vivo recycling, we selected for 2 variant cell lines: 253J B-V, a bladder line isolated after 5 serial passages in the bladder, and 253J lung-IV, established from a lung tumor nodule that was recycled through the bladder.
These 2 cell lines showed enhanced tumorigenicity, as measured by a decreased latent period, and rapid growth as compared with the parental cell line. Moreover, orthotopic implantation of these cell lines resulted in metastasis to the lungs. These in vivo-selected, metastatic cell lines exhibited unique karyotypic alterations, increased anchorage-independent growth, overexpression of basic fibroblast growth factor, altered expression of adhesion molecules and the ability to migrate through Matrigel.
This reproducible model of human bladder cancer offers the opportunity to study cellular properties associated with tumor progression and metastasis and is suitable for the evaluation of new therapeutic strategies for invasive bladder cancer.
这些研究旨在建立一种人膀胱癌原位模型并分离转移性变异细胞系。
将人膀胱癌细胞系253J植入无胸腺裸鼠膀胱的肌层。通过体内循环，我们筛选出2个变异细胞系：253J B-V，在膀胱中连续传代5次后分离得到的膀胱细胞系；以及253J lung-IV，由通过膀胱循环的肺肿瘤结节建立。
与亲代细胞系相比，这2个细胞系的致瘤性增强，表现为潜伏期缩短，生长迅速。此外，这些细胞系的原位植入导致肺转移。这些体内筛选出的转移性细胞系表现出独特的核型改变、非贴壁依赖性生长增加、碱性成纤维细胞生长因子过表达、黏附分子表达改变以及穿过基质胶迁移的能力。
这种可重复的人膀胱癌模型为研究与肿瘤进展和转移相关的细胞特性提供了机会，适用于评估侵袭性膀胱癌的新治疗策略。
