Hennings Leah J, Flores Rene, Roberson Paula K, Brown Aliza, Lowery John, Borrelli Michael, Culp William C
Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Stroke Res Treat. 2011;2011:764830. doi: 10.4061/2011/764830. Epub 2011 Jul 3.
Duration and extent of penumbra determine the window and brain volume in which interventions may save injured tissue after stroke. Understanding the penumbra in animals is necessary in order to design models that translate to effective clinical therapies. New Zealand white rabbits were embolized with aged autologous clot (n = 23) or insoluble microspheres (n = 21). To examine effects of treatment on penumbra, sphere-stroked animals were treated with 3 μm microbubbles plus ultrasound (n = 19). Rabbits were euthanized at 4 or 24 hr. Infarct volume was measured following triphenyltetrazolium chloride (TTC) staining of brain sections. Penumbra was visualized using immunostaining of pimonidazole injected fifteen minutes prior to euthanasia. Potentially reversible penumbra was present in 14.3% stroked rabbits at 4 hours and 15.7% at 24 hours after embolic stroke and represented up to 35% of total lost tissue. Intervention at up to 24 hours may benefit a significant patient population.
半暗带的持续时间和范围决定了中风后干预措施可能挽救受损组织的时间窗和脑容量。为了设计能够转化为有效临床治疗方法的模型，了解动物体内的半暗带是很有必要的。将新西兰白兔用陈旧的自体血凝块（n = 23）或不溶性微球（n = 21）进行栓塞。为了研究治疗对半暗带的影响，对用微球栓塞的动物用3μm微泡加超声进行治疗（n = 19）。在4小时或24小时时对兔子实施安乐死。对脑切片进行氯化三苯基四氮唑（TTC）染色后测量梗死体积。在安乐死前15分钟注射匹莫硝唑，通过免疫染色观察半暗带。栓塞性中风后4小时，14.3%的中风兔子存在潜在可逆性半暗带，24小时时为15.7%，且占总损失组织的比例高达35%。长达24小时的干预可能使相当一部分患者受益。
