Department of Radiology, 4301 West Markham Street, University of Arkansas for Medical Sciences, Little Rock, AR 72205-7199, USA.
J Neurosci Methods. 2013 Jan 30;212(2):322-8. doi: 10.1016/j.jneumeth.2012.10.017. Epub 2012 Nov 8.
To develop angiographic models of embolic stroke in the rabbit using pre-formed clot or microspheres to model clinical situations ranging from transient ischemic events to severe ischemic stroke.
New Zealand White rabbits (N=151) received angiographic access to the internal carotid artery (ICA) from a femoral approach. Variations of emboli type and quantity of emboli were tested by injection into the ICA. These included fresh clots (1.0-mm length, 3-6h), larger aged clots (4.0-mm length, 3 days), and 2 or 3 insoluble microspheres (700-900 μm). Neurological assessment scores (NAS) were based on motor, sensory, balance, and reflex measures. Rabbits were euthanized at 4, 7, or 24h after embolization, and infarct volume was measured as a percent of total brain volume using 2,3,5-triphenyltetrazolium chloride (TTC).
Infarct volume percent at 24 h after stroke was lower for rabbits embolized with fresh clot (0.45±0.14%), compared with aged clot (3.52±1.31%) and insoluble microspheres (3.39±1.04%). Overall NAS (including posterior vessel occlusions) were positively correlated to infarct volume percent measurements in the fresh clot (r=0.50), aged clot (r=0.65) and microsphere (r=0.62) models (p<0.001).
The three basic angiographic stroke models may be similar to human transient ischemic attacks (TIA) (fresh clot), major strokes that can be thrombolysed (aged clot), or major strokes with insoluble emboli such as atheromata (microspheres). Model selection can be tailored to specific research needs.
使用预先形成的血栓或微球在兔体内建立血管造影栓塞性中风模型，以模拟从短暂性缺血事件到严重缺血性中风等临床情况。
新西兰白兔（N=151）通过股动脉入路获得颈内动脉（ICA）的血管造影通道。通过向 ICA 内注射来测试栓塞类型和栓塞数量的变化。这些包括新鲜血栓（1.0mm 长，3-6h）、较大的陈旧血栓（4.0mm 长，3 天）和 2 或 3 个不溶性微球（700-900μm）。神经评估评分（NAS）基于运动、感觉、平衡和反射测量。栓塞后 4、7 或 24 小时处死兔子，并使用 2,3,5-三苯基氯化四唑（TTC）测量梗塞体积占总脑体积的百分比。
栓塞后 24 小时，新鲜血栓组（0.45±0.14%）的梗塞体积百分比低于陈旧血栓组（3.52±1.31%）和不溶性微球组（3.39±1.04%）。总的 NAS（包括后血管闭塞）与新鲜血栓（r=0.50）、陈旧血栓（r=0.65）和微球（r=0.62）模型中的梗塞体积百分比测量值呈正相关（p<0.001）。
这三种基本的血管造影性中风模型可能类似于人类短暂性缺血发作（TIA）（新鲜血栓）、可溶栓的大中风（陈旧血栓）或不溶性栓子如动脉粥样硬化（微球）引起的大中风。模型选择可以根据特定的研究需求进行调整。
