Nishida A, Kimura H, Sugioka K, Nakano M
Department of Neonatology, Gunma Children's Medical Center, Hokkitsu, Japan.
Biol Neonate. 1990;58(3):145-51. doi: 10.1159/000243254.
The ability of granulocytes to generate superoxide anions (O2-) and hypochlorite (OCl-) during phagocytosis was investigated using peripheral blood samples from adults and cord blood samples from neonates, using the chemiluminescence probe cypiridina luciferin analog (CLA) for O2- generation and luminol (L) for OCl- generation. OCl- generation by granulocytes was also monitored by taurine chloramine formation. The chemiluminescence probe based upon CLA was highly specific for and sensitive to O2- and could be adopted to determine O2- generation in terms of xanthine oxidase units. The CLA-dependent chemiluminescence by cord blood granulocytes was significantly higher than that by normal adult granulocytes. Taurine chloramine formation was significantly correlated with the L-dependent chemiluminescence (L-CL). Thus, the L-CL is considered to be mainly involved in OCl- generated by phagocytizing granulocytes. L-CLs by cord blood granulocytes and normal adult granulocytes were essentially the same during phagocytosis.
利用来自成年人的外周血样本和来自新生儿的脐血样本，使用化学发光探针海萤荧光素类似物（CLA）检测吞噬作用期间粒细胞产生超氧阴离子（O2-）的能力，使用鲁米诺（L）检测产生次氯酸盐（OCl-）的能力。粒细胞产生OCl-的情况也通过牛磺酸氯胺的形成进行监测。基于CLA的化学发光探针对O2-具有高度特异性和敏感性，可用于根据黄嘌呤氧化酶单位测定O2-的产生。脐血粒细胞依赖CLA的化学发光显著高于正常成年人粒细胞。牛磺酸氯胺的形成与依赖L的化学发光（L-CL）显著相关。因此，L-CL被认为主要参与吞噬粒细胞产生的OCl-。在吞噬作用期间，脐血粒细胞和正常成年人粒细胞的L-CL基本相同。
