Sugioka K, Nakano M, Kurashige S, Akuzawa Y, Goto T
FEBS Lett. 1986 Mar 3;197(1-2):27-30. doi: 10.1016/0014-5793(86)80291-5.
When a Cypridina luciferin analog (the title compound) was added to a macrophage suspension in Hank's balanced salt solution (control), the system emitted a weak, but detectable light, which was not altered in the presence of superoxide dismutase. The same system, however, emitted a much stronger light, just after the addition of a trigger, opsonized zymosan. The luminescence was suppressed to the control level in the presence of superoxide dismutase, while it was only slightly influenced, if at all, by NaN3, a scavenger of singlet oxygen and an inhibitor of myeloperoxidase. Some other results obtained also indicate the participation of O2- in the luciferin analog-dependent luminescence in macrophages during phagocytosis.
当将一种海萤荧光素类似物（标题化合物）添加到汉克平衡盐溶液中的巨噬细胞悬液（对照）中时，该系统发出微弱但可检测到的光，在超氧化物歧化酶存在的情况下该光没有变化。然而，在添加引发剂（调理酵母聚糖）后，同一系统发出更强的光。在超氧化物歧化酶存在下，发光被抑制到对照水平，而NaN₃（单线态氧清除剂和髓过氧化物酶抑制剂）对其影响甚微（如果有影响的话）。获得的其他一些结果也表明，在吞噬过程中，O₂⁻参与了巨噬细胞中依赖荧光素类似物的发光过程。
