Assali Mohyeddin, Zaid Abdel Naser, Abdallah Farah, Almasri Motasem, Khayyat Rasha
Department of Pharmacy, Faculty of Medicine and Health Sciences, An Najah National University, Nablus, Palestine.
Department of Biology and Biotechnology, Faculty of Science, An Najah National University, Nablus, Palestine.
Int J Nanomedicine. 2017 Sep 7;12:6647-6659. doi: 10.2147/IJN.S140625. eCollection 2017.
As infectious diseases continue to be one of the greatest health challenges worldwide, the demand toward alternative agents is continuously increasing. Recent advancement in nanotechnology has expanded our ability to design and construct nanomaterials to treat bacterial infections. Carbon nanotubes are one among these nanomaterials. Herein, we describe the covalent functionalization of the single-walled carbon nanotubes (SWCNTs) with multiple molecules of ciprofloxacin. The prepared nanoantibiotics were characterized using different techniques, including transmission electron microscopy, Raman spectroscopy, and thermogravimetric analysis. The characterization of the nanoantibiotics confirmed the successful covalent functionalization of the SWCNTs with 55% of functionalization as has been observed by thermogravimetric analysis. The release profile revealed that 90% of the loaded ciprofloxacin was released within 2.5 h at pH 7.4 showing a first-order release profile with >0.99. Interestingly, the results of the antibacterial activity indicated that the functionalized SWCNTs have significant increase in the antibacterial activity against the three strains of bacteria - by 16-fold for and and by 8-fold for - in comparison to the ciprofloxacin free drug. Moreover, the synthesized nanoantibiotic showed high hemocompatibility and cytocompatibility over a wide concentration range.
由于传染病仍然是全球最大的健康挑战之一，对替代药物的需求持续增长。纳米技术的最新进展扩展了我们设计和构建用于治疗细菌感染的纳米材料的能力。碳纳米管就是这些纳米材料之一。在此，我们描述了用多个环丙沙星分子对单壁碳纳米管（SWCNT）进行共价功能化。使用不同技术对制备的纳米抗生素进行了表征，包括透射电子显微镜、拉曼光谱和热重分析。纳米抗生素的表征证实了SWCNT的成功共价功能化，热重分析观察到功能化率为55%。释放曲线显示，在pH 7.4时，90%负载的环丙沙星在2.5小时内释放，呈现出大于0.99的一级释放曲线。有趣的是，抗菌活性结果表明，与游离环丙沙星药物相比，功能化的SWCNT对三种细菌菌株的抗菌活性显著增加——对[具体菌株1]增加了16倍，对[具体菌株2]增加了16倍，对[具体菌株3]增加了8倍。此外，合成的纳米抗生素在很宽的浓度范围内显示出高血液相容性和细胞相容性。
