Department of Pharmacology, University of Bologna, Bologna, Italy.
Mutat Res. 2011 Sep 1;714(1-2):88-92. doi: 10.1016/j.mrfmmm.2011.06.016. Epub 2011 Jul 14.
Oxidative damage plays an important role in the pathogenesis of colorectal (CR) cancer. This study investigated the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S-transferase (GST) in plasma of 82 participants of a screening program for CR cancer prevention (30 females and 52 males; age 50-70 years). All subjects resulted positive to fecal occult blood test and were subsequently classified, according to the colonoscopy and histological findings, in patients with CR cancer, patients with colorectal polyps or controls. Furthermore, the activity of clastogenic factors (CFs) in plasma from study population was measured as the ability of inducing micronuclei (MN) in vitro in peripheral of a healthy donor. CAT and GR activities were significantly lower in CR cancer patients compared to controls (P<0.05) and polyps groups (P<0.05). SOD activity was significantly higher in patients with CR cancer than in polyp (P<0.05) and control (P<0.05) groups. GST activity was not significantly different in plasma of the three groups. An increase of CFs induction was observed in plasma of CR cancer patients (MN: 8.89±3.42) with respect to control (MN: 6.37±0.96 P<0.05). These results can contribute to define plasma biomarkers associated to oxidative stress damage that could predictive of CR cancer risk.
氧化损伤在结直肠癌（CR）的发病机制中起着重要作用。本研究调查了抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和谷胱甘肽-S-转移酶（GST）在 82 名 CR 癌症预防筛查计划参与者（30 名女性和 52 名男性；年龄 50-70 岁）血浆中的活性。所有受试者粪便潜血试验均呈阳性，随后根据结肠镜和组织学检查结果，将受试者分为 CR 癌症患者、结直肠息肉患者或对照组。此外，还测量了研究人群血浆中致裂因素（CFs）的活性，作为体外诱导健康供体外周微核（MN）的能力。与对照组（P<0.05）和息肉组（P<0.05）相比，CR 癌症患者的 CAT 和 GR 活性明显降低。SOD 活性在 CR 癌症患者中明显高于息肉（P<0.05）和对照组（P<0.05）组。三组血浆中 GST 活性无明显差异。CR 癌症患者血浆中 CFs 诱导增加（MN：8.89±3.42）与对照组（MN：6.37±0.96，P<0.05）。这些结果有助于确定与氧化应激损伤相关的血浆生物标志物，这些标志物可能预测 CR 癌症风险。
