用于检测人疱疹病毒6型IgG抗体的酶联免疫吸附测定

Enzyme-linked immunosorbent assay for detection of IgG antibody to human herpesvirus 6.

作者信息

Asano Y, Yoshikawa T, Suga S, Yazaki T, Ozaki T, Saito Y, Hatano Y, Takahashi M

机构信息

Department of Pediatrics, Fujita Health University School of Medicine, Aichi, Japan.

出版信息

J Med Virol. 1990 Oct;32(2):119-23. doi: 10.1002/jmv.1890320209.

DOI:10.1002/jmv.1890320209

PMID:2177773

Abstract

A lysate of human herpesvirus 6 (HHV-6)-infected cord blood mononuclear cells was used as antigen for enzyme-linked immunosorbent assay for the detection of IgG antibody to HHV-6. Antibody responses after exanthem subitum were well correlated with clinical recovery from the disease and the level of antibody activities was well correlated with indirect immunofluorescence assay and the neutralization test. Seroconversion to other human herpesvirus, including cytomegalovirus, was not observed in infants with exanthem subitum. All of the infants had by the age of 1 month antibodies to HHV-6, which decreased with age to the lowest level at the age of 3 to 6 months and then increased and reached the maximum level by 1 to 2 years of age. After 3 years of age, the prevalence was almost stable.

摘要

用人疱疹病毒6型（HHV-6）感染的脐血单个核细胞裂解物作为抗原，采用酶联免疫吸附试验检测HHV-6 IgG抗体。幼儿急疹后的抗体反应与疾病的临床恢复情况密切相关，抗体活性水平与间接免疫荧光试验及中和试验密切相关。患幼儿急疹的婴儿未观察到向包括巨细胞病毒在内的其他人类疱疹病毒的血清学转换。所有婴儿在1月龄时均有HHV-6抗体，该抗体随年龄增长而下降，在3至6月龄时降至最低水平，随后升高，在1至2岁时达到最高水平。3岁以后，患病率几乎稳定。