Cermelli C, Moroni A, Montorsi M, Fabio G, Portolani M
Institute of Hygiene, University of Modena, Italy.
New Microbiol. 1994 Apr;17(2):69-73.
546 human sera from healthy subjects, subjects with cytomegalovirus or Epstein-Barr virus infection and from cordal blood were tested for the human herpesvirus-6 (HHV-6) IgG content in an enzyme-linked immunosorbent assay (ELISA) to verify results previously obtained with an immunofluorescence assay (IFA). The HHV-6 isolate CV, obtained from a child with exanthem subitum, employed as antigen in both tests, belonged to HHV-6 variant B. The ELISA results were similar to those obtained with IFA. 445/546 (81.5%) sera had the same type of reactivity with the two serological procedures. To establish whether different HHV-6 strains may give different serological results, 117 out of the 546 sera tested with IFA against CV infected cells were also tested against cells infected with the HHV-6 isolate U1102 belonging to variant A. All the sera showed a similar reactivity.
对来自健康受试者、巨细胞病毒或爱泼斯坦-巴尔病毒感染受试者以及脐带血的546份人血清，采用酶联免疫吸附测定（ELISA）检测人疱疹病毒6型（HHV-6）IgG含量，以验证先前通过免疫荧光测定（IFA）获得的结果。从一名幼儿急疹患儿分离得到的HHV-6毒株CV，在两种检测中均用作抗原，属于HHV-6 B型变种。ELISA结果与IFA结果相似。445/546（81.5%）的血清在两种血清学检测方法中具有相同类型的反应性。为确定不同的HHV-6毒株是否会产生不同的血清学结果，对546份用IFA检测CV感染细胞的血清中的117份，也检测了其对属于A型变种的HHV-6毒株U1102感染细胞的反应性。所有血清均显示出相似的反应性。
