Karaca K, Naqi S A, Palukaitis P, Lucio B
Department of Avian and Aquatic Animal Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Avian Dis. 1990 Oct-Dec;34(4):899-904.
Three coronaviruses isolated from the intestines of laying chickens were partially characterized. Serological and molecular assays indicated that the enteric coronaviruses are infectious bronchitis virus (IBV) isolates. Although the three isolates were recovered from three unrelated chicken flocks, their RNase T1-resistant oligonucleotide fingerprints were almost identical. The three isolates were not neutralized by antisera specific to IBV serotype Connecticut, but their RNase T1-resistant oligonucleotide fingerprints closely matched the fingerprints of strain Conn-46, a Connecticut serotype. This and the co-fingerprinting data suggested that the three field isolates may have emerged from the Connecticut virus through mutation(s). The mutation(s) apparently involved the S1 protein gene that determines the virus serotype.
对从蛋鸡肠道中分离出的三种冠状病毒进行了部分特性鉴定。血清学和分子检测表明，这些肠道冠状病毒为传染性支气管炎病毒（IBV）分离株。尽管这三种分离株分别从三个不相关的鸡群中获得，但它们的核糖核酸酶T1抗性寡核苷酸指纹图谱几乎相同。这三种分离株不能被IBV康涅狄格血清型特异性抗血清中和，但其核糖核酸酶T1抗性寡核苷酸指纹图谱与康涅狄格血清型毒株Conn-46的指纹图谱非常匹配。这一点以及共同指纹图谱数据表明，这三种现场分离株可能是通过突变从康涅狄格病毒演变而来。这种突变显然涉及决定病毒血清型的S1蛋白基因。
