Zaman Md Badruz, Baral Toya Nath, Jakubek Zygmunt J, Zhang Jianbing, Wu Xiaohua, Lai Edward, Whitfield Dennis, Yu Kui
Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
J Nanosci Nanotechnol. 2011 May;11(5):3757-63. doi: 10.1166/jnn.2011.4167.
Successful targeted imaging of BxPC3 human pancreatic cancer cells is feasible with near-IR CdTeSe/CdS quantum dots (QDs) functionalized with single-domain antibody (sdAb) 2A3. For specific targeting, sdAbs are superior to conventional antibodies, especially in terms of stability, aggregation, and production cost. The bright CdTeSe/CdS QDs were synthesized to emit in the diagnostic window of 650-900 nm with a narrow emission band. 2A3 was derived from llama and is small in size of 13 kDa, but with fully-functional recognition to the target carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), a possible biomarker as a therapeutic target of pancreatic cancer. For compelling imaging, optical may be the most sensible among the various imaging modalities, regarding the sensitivity and cost. This first report on sdAb-conjugated near-IR QDs with high signal to background sensitivity for targeted cellular imaging brings insights into the development of optical molecular imaging for early stage cancer diagnosis.
使用用单域抗体（sdAb）2A3功能化的近红外碲化镉硒/硫化镉量子点（QD）对BxPC3人胰腺癌细胞进行成功的靶向成像具有可行性。对于特异性靶向，单域抗体优于传统抗体，尤其是在稳定性、聚集性和生产成本方面。合成了明亮的碲化镉硒/硫化镉量子点，使其在650-900nm的诊断窗口内发射，发射带较窄。2A3源自羊驼，大小为13kDa，但对靶标癌胚抗原相关细胞粘附分子6（CEACAM6）具有完全功能性识别，CEACAM6是一种可能作为胰腺癌治疗靶点的生物标志物。对于引人注目的成像，就灵敏度和成本而言，光学成像可能是各种成像方式中最明智的。这篇关于具有高信号背景灵敏度的单域抗体偶联近红外量子点用于靶向细胞成像的首次报告为早期癌症诊断的光学分子成像发展带来了见解。
