Kim Min-Jung, Jang Dae-Hwan, Kim Hak-Kyong, Lee Young-In, Lee Gun-Jae, Yoo Bong-Young, Choa Yong-Ho
Department of Chemical Engineering, Hanyang University, Ansan 426-791, Korea.
J Nanosci Nanotechnol. 2011 May;11(5):4592-5. doi: 10.1166/jnn.2011.3638.
Liposome encapsulating Fe3O4 (liposome complexes) has been prepared for targeting a drug to a specific organ, as well as for MRI (magnetic resonance imaging) contrast agents. The objective of the present work was to investigate the Fe3O4 properties and the effects of chitosan concentration on the characteristics of chitosan-coated liposome complexes. They were characterized by DLS, FT-IR, XRD, VSM, UV-Vis spectrometer, TEM and phase-contrast microscopy. The average liposome complex size was approximately 500 nm, with individual Fe3O4 nanoparticle sizes of 10 nm. The drug incorporation efficiency of trypsin in liposome complexes was 65-69%, the drug release was sustained and the incorporated drugs had the magnetization properties of the liposome complexes. Incorporation of chitosan into the liposome bilayer decreased trypsin release from the liposome complexes due to an increased rigidity of the liposome membrane structure. Chitosan-coated liposome complexes showed a higher stability when compared with the stability of non-coated liposome complexes.
用于将药物靶向特定器官以及作为磁共振成像（MRI）造影剂的包裹四氧化三铁的脂质体（脂质体复合物）已被制备出来。本研究的目的是研究四氧化三铁的性质以及壳聚糖浓度对壳聚糖包被脂质体复合物特性的影响。通过动态光散射（DLS）、傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、振动样品磁强计（VSM）、紫外可见光谱仪、透射电子显微镜（TEM）和相差显微镜对它们进行了表征。脂质体复合物的平均尺寸约为500纳米，单个四氧化三铁纳米颗粒的尺寸为10纳米。胰蛋白酶在脂质体复合物中的药物包封率为65%-69%，药物释放是持续的，且包封的药物具有脂质体复合物的磁化特性。壳聚糖掺入脂质体双层中会降低胰蛋白酶从脂质体复合物中的释放，这是由于脂质体膜结构的刚性增加所致。与未包被的脂质体复合物相比，壳聚糖包被的脂质体复合物表现出更高的稳定性。
