Kim Min-Jung, Jang Dae-Hwan, Lee Young-In, Jung Hyun Sook, Lee Hak-Jong, Choa Yong-Ho
Department of Chemical Engineering, Hanyang University, 1271 Sa-1, Ansan, Gyeonggi-do 426-791, Korea.
J Nanosci Nanotechnol. 2011 Jan;11(1):889-93. doi: 10.1166/jnn.2011.3267.
Phospholipid vesicles encapsulating magnetic nanoparticles (liposome complexes) have been prepared for targeting a drug to a specific organ using a magnetic force, as well as for local hyperthermia therapy. Liposome complexes are also an ideal platform for use as contrast agents of magnetic resonance imaging (MRI). We describe the preparation and characterization of liposomes containing magnetite. These liposomes were obtained by thin film hydration method and Fe3O4 nanoparticles were synthesized by coprecipitation method. They were characterized by an electrophoretic light scattering spectrophotometer, the liposome complexes were subsequently coated using chitosan. We have further investigated the ability of the above formulation for drug delivery and MRI applications. We are specifically interested in evaluating our liposome complexes for drug therapy; hence, we selected paclitaxel for the combination study. The amount of paclitaxel was measured at 227 nm using a UV-Vis spectrophotometer. Cytotoxicity of liposome complexes was treated with the various concentrations of paclitaxel in PC3 cell lines. The structure and properties of liposome complexes were analyzed by FT-IR, XRD and VSM. The particle size was analyzed by TEM and DLS.
已制备出包裹磁性纳米颗粒的磷脂囊泡（脂质体复合物），用于利用磁力将药物靶向特定器官，以及用于局部热疗。脂质体复合物也是用作磁共振成像（MRI）造影剂的理想平台。我们描述了含磁铁矿脂质体的制备和表征。这些脂质体通过薄膜水化法获得，Fe3O4纳米颗粒通过共沉淀法合成。用电泳光散射分光光度计对其进行表征，随后用壳聚糖包被脂质体复合物。我们进一步研究了上述制剂用于药物递送和MRI应用的能力。我们特别感兴趣的是评估我们的脂质体复合物用于药物治疗；因此，我们选择紫杉醇进行联合研究。使用紫外可见分光光度计在227nm处测量紫杉醇的含量。用不同浓度的紫杉醇处理脂质体复合物在PC3细胞系中的细胞毒性。通过傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）和振动样品磁强计（VSM）分析脂质体复合物的结构和性质。通过透射电子显微镜（TEM）和动态光散射（DLS）分析粒径。
