Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
J Agric Food Chem. 2011 Aug 24;59(16):8609-15. doi: 10.1021/jf2015528. Epub 2011 Jul 29.
The present study was to characterize a procyanidin-rich extract (PARE) from sorghum ( Sorghum bicolor (L.) Moench) bran and assess its biological activities. The procyanidin oligomers were separated and identified by normal-phase HPLC equipped with fluorescence (FLD) and mass spectrometry (MS) detectors. In addition, the effects of PARE on oxidative stress in mice induced by D-galactose as well as tumor inhibition in C57BL/6J mice bearing Lewis lung cancer were investigated. Administration of D-galactose significantly (p < 0.05) lowered the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). This was accompanied by a significant (p < 0.05) increase in malondialdehyde (MDA) levels in both liver and serum. Administration of PARE (150 mg/kg) significantly (p < 0.05) reversed the d-galactose-induced oxidative stress by enhancing the activities of antioxidant enzymes. Furthermore, PARE administration inhibited tumor growth and metastasis formation by suppressing vascular endothelial growth factor (VEGF) production. The results suggested that PARE had antioxidant and antitumor activities.
本研究旨在对高粱糠中原花青素丰富提取物（PARE）进行表征，并评估其生物活性。采用配备荧光（FLD）和质谱（MS）检测器的正相 HPLC 对原花青素低聚物进行分离和鉴定。此外，还研究了 PARE 对 D-半乳糖诱导的小鼠氧化应激以及对携带 Lewis 肺癌的 C57BL/6J 小鼠的肿瘤抑制作用。D-半乳糖给药显著（p < 0.05）降低了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。这伴随着肝和血清中丙二醛（MDA）水平的显著（p < 0.05）增加。PARE（150mg/kg）给药通过增强抗氧化酶的活性，显著（p < 0.05）逆转了 D-半乳糖诱导的氧化应激。此外，PARE 给药通过抑制血管内皮生长因子（VEGF）的产生来抑制肿瘤生长和转移形成。结果表明，PARE 具有抗氧化和抗肿瘤活性。
