Lone Irshad A, Kaur Gurpreet, Athar Mohammad, Alam M Sarwar
Department of Chemistry, Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110062, India.
Food Chem Toxicol. 2007 Oct;45(10):1821-9. doi: 10.1016/j.fct.2007.03.021. Epub 2007 Apr 19.
In this communication, we document the antioxidant potential of ethanolic extract of Rumex patientia L. (Polygonaceae) roots and its chemopreventive effects against Fe-NTA mediated hepatic oxidative stress, hepatotoxicity and tumor promotion response. The extract exhibited high polyphenolic content, potent reducing power and significantly scavenged free radicals (including several reactive oxygen species (ROS) and reactive nitrogen species (RNS)). The extract also significantly and dose dependently protected against oxidative damage to lipids and DNA. These results indicated R. patientia root extract to exert a potent antioxidant activity in vitro. The efficacy of extract was also evaluated in vivo and it was found to exert a potent protective affect in acute oxidative tissue injury animal model: ferric nitrilotriacetate (Fe-NTA) induced hepatotoxicity in mice. Administration of Fe-NTA (9 mg/kg body weight, i.p.) to mice led to a significant oxidative stress and allied damage in liver tissues and induced hyperproliferation. A significant depletion was observed in GSH content and enzymes implicated in its metabolism. Attenuation also occurred in activities of other hepatic antioxidant enzymes including SOD, CAT, and GPX. Fe-NTA also incited hyperproliferation response elevating ornithine decarboxylase activity and [(3)H]-thymidine incorporation into DNA. Histopathological investigations and liver function tests (LFT) indicated Fe-NTA to cause extensive hepatic damage. However, prophylactic treatment with R. patientia root extract at a dose regimen of 100-200mg/kg body weight for a week not only restored hepatic antioxidant armory close to normal, but also significantly precluded oxidative damage restoring normal hepatic architecture and levels of hepatic damage markers. The data obtained in the present study illustrates R. patientia roots to possess potent antioxidant and free radical scavenging activities and thwart oxidative damage and hyperproliferation in hepatic tissues.
在本报告中，我们记录了皱叶酸模（蓼科）根乙醇提取物的抗氧化潜力及其对铁-次氮基三乙酸（Fe-NTA）介导的肝脏氧化应激、肝毒性和肿瘤促进反应的化学预防作用。该提取物表现出高多酚含量、强大的还原能力，并能显著清除自由基（包括多种活性氧（ROS）和活性氮（RNS））。该提取物还能显著且呈剂量依赖性地保护脂质和DNA免受氧化损伤。这些结果表明皱叶酸模根提取物在体外具有强大的抗氧化活性。还对提取物在体内的功效进行了评估，发现在急性氧化组织损伤动物模型（Fe-NTA诱导的小鼠肝毒性）中，它具有强大的保护作用。给小鼠腹腔注射Fe-NTA（9 mg/kg体重）会导致肝脏组织出现显著的氧化应激及相关损伤，并诱导细胞过度增殖。谷胱甘肽（GSH）含量及其代谢相关酶出现显著消耗。包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）在内的其他肝脏抗氧化酶的活性也有所降低。Fe-NTA还引发细胞过度增殖反应，提高鸟氨酸脱羧酶活性并使[³H]胸腺嘧啶掺入DNA。组织病理学研究和肝功能测试（LFT）表明Fe-NTA会导致广泛的肝脏损伤。然而，以100 - 200mg/kg体重的剂量方案对小鼠进行一周的皱叶酸模根提取物预防性治疗，不仅能使肝脏抗氧化防御系统恢复至接近正常水平，还能显著防止氧化损伤，恢复正常的肝脏结构和肝脏损伤标志物水平。本研究获得的数据表明皱叶酸模根具有强大的抗氧化和自由基清除活性，并能防止肝脏组织的氧化损伤和细胞过度增殖。
