Department of Biological and Physiological Sciences, Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura, Universidad Peruana Cayetano Heredia , Lima , Peru.
Toxicol Mech Methods. 2011 Oct;21(8):628-34. doi: 10.3109/15376516.2011.583294. Epub 2011 Jul 22.
Previous studies have shown that black variety of maca has beneficial effects on learning and memory in experimental animal models. The present study aimed to determine whether the hydroalcoholic extract of black maca (BM) showed a dose-response effect in mice treated with ethanol 20% (EtOH) as a model of memory impairment. Mice were divided in the following groups: control, EtOH, ascorbic acid (AA) and 0.125, 0.25, 0.50 and 1.00 g/kg of BM plus EtOH. All treatments were orally administered for 28 days. Open field test was performed to determine locomotor activity and water Morris maze was done to determine spatial memory. Also, total polyphenol content in the hydroalcoholic extract of BM was determined (0.65 g pyrogallol/100 g). Mice treated with EtOH took more time to find the hidden platform than control during escape acquisition trials; meanwhile, AA and BM reversed the effect of EtOH. In addition, AA and BM ameliorated the deleterious effect of EtOH during the probe trial. Correlation analyses showed that the effect of BM a dose-dependent behavior. Finally, BM improved experimental memory impairment induced by ethanol in a dose-response manner due, in part, to its content of polyphenolic compounds.
先前的研究表明，黑玛卡品种对实验动物模型的学习和记忆具有有益影响。本研究旨在确定黑玛卡（BM）的水醇提取物是否对乙醇 20%（EtOH）处理的小鼠表现出剂量反应效应，作为记忆障碍的模型。将小鼠分为以下几组：对照组、EtOH 组、抗坏血酸（AA）组和 0.125、0.25、0.50 和 1.00 g/kg 的 BM+EtOH 组。所有治疗均经口给予 28 天。通过旷场试验测定运动活性，通过水迷宫试验测定空间记忆。还测定了 BM 水醇提取物中的总多酚含量（100 g 没食子儿茶素中含有 0.65 g 焦儿茶酚）。与对照组相比，接受 EtOH 处理的小鼠在逃避获得试验中找到隐藏平台需要更长的时间；而 AA 和 BM 逆转了 EtOH 的作用。此外，AA 和 BM 在探测试验中改善了 EtOH 的有害作用。相关性分析表明，BM 的作用呈剂量依赖性。最后，BM 以剂量反应的方式改善了乙醇引起的实验性记忆障碍，这部分归因于其多酚化合物的含量。
