Molecular Virology Laboratory, Rajiv Gandhi Centre for Biotechnology (RGCB), Kerala, India.
Virol J. 2011 Jul 23;8:363. doi: 10.1186/1743-422X-8-363.
The human hepatitis B virus (HBV), a member of the hepadna viridae, causes acute or chronic hepatitis B, and hepatocellular carcinoma (HCC). The duck hepatitis B virus (DHBV) infection, a dependable and reproducible model for hepadna viral studies, does not result in HCC unlike chronic HBV infection. Information on differential gene expression in DHBV infection might help to compare corresponding changes during HBV infection, and to delineate the reasons for this difference.
A subtractive hybridization cDNA library screening of in vitro DHBV infected, cultured primary duck hepatocytes (PDH) identified cDNAs of 42 up-regulated and 36 down-regulated genes coding for proteins associated with signal transduction, cellular respiration, transcription, translation, ubiquitin/proteasome pathway, apoptosis, and membrane and cytoskeletal organization. Those coding for both novel as well as previously reported proteins in HBV/DHBV infection were present in the library. An inverse modulation of the cDNAs of ten proteins, reported to play role in human HCC, such as that of Y-box binding protein1, Platelet-activating factor acetylhydrolase isoform 1B, ribosomal protein L35a, Ferritin, α-enolase, Acid α-glucosidase and Caspase 3, copper-zinc superoxide dismutase (CuZnSOD), Filamin and Pyruvate dehydrogenase, was also observed in this in vitro study.
The present study identified cDNAs of a number of genes that are differentially modulated in in vitro DHBV infection of primary duck hepatocytes. Further correlation of this differential gene expression in in vivo infection models would be valuable to understand the little known aspects of the hepadnavirus biology.
乙型肝炎病毒（HBV）是嗜肝 DNA 病毒科的一员，可引起急性或慢性乙型肝炎和肝细胞癌（HCC）。鸭乙型肝炎病毒（DHBV）感染是一种可靠且可重现的嗜肝 DNA 病毒研究模型，与慢性 HBV 感染不同，它不会导致 HCC。有关 DHBV 感染中差异基因表达的信息可能有助于比较 HBV 感染过程中的相应变化，并阐明这种差异的原因。
体外 DHBV 感染培养的原代鸭肝细胞（PDH）的消减杂交 cDNA 文库筛选鉴定了 42 个上调和 36 个下调基因的 cDNA，这些基因编码与信号转导、细胞呼吸、转录、翻译、泛素/蛋白酶体途径、细胞凋亡以及膜和细胞骨架组织相关的蛋白质。该文库中存在编码在 HBV/DHBV 感染中既有新蛋白也有先前报道过的蛋白的 cDNA。十种在人类 HCC 中发挥作用的蛋白质的 cDNA 呈相反的调节方式，如 Y 框结合蛋白 1、血小板激活因子乙酰水解酶同工酶 1B、核糖体蛋白 L35a、铁蛋白、α-烯醇酶、酸性α-葡萄糖苷酶和 Caspase 3、铜锌超氧化物歧化酶（CuZnSOD）、细丝蛋白和丙酮酸脱氢酶。在这项体外研究中也观察到了这种情况。
本研究鉴定了在原代鸭肝细胞体外 DHBV 感染中差异调节的一些基因的 cDNA。在体内感染模型中进一步相关这种差异基因表达将有助于了解嗜肝 DNA 病毒生物学的一些未知方面。
