Orthopedic Clinic, University of Ferrara, Ferrara, Italy.
Int J Immunopathol Pharmacol. 2011 Apr-Jun;24(2 Suppl):51-7. doi: 10.1177/03946320110240S210.
Calcium sulfate (CaS) is a highly biocompatible material and enhances bone formation in vivo. However, how CaS alters osteoblast activity to promote bone formation is poorly understood. To study how CaS can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in normal osteoblasts and dental pulp stem cells, using real time Reverse Transcription-Polymerase Chain Reaction. Gene differentially expressed between the two cells type were the trascriptional factor RUNX2, osteopontin (SPP1), COL1A1 (collagen type 1α1) and alkaline phosphatase (ALPL). The obtained results demonstrated that CaS strongly influences the behavior of DPSCs in vitro enhancing proliferation, differentiation and deposition of matrix.
硫酸钙（CaS）是一种高度生物相容性材料，可在体内增强成骨作用。但是，尚不清楚 CaS 如何改变成骨细胞活性以促进骨形成。为了研究 CaS 如何诱导间充质干细胞中的成骨细胞分化，使用实时逆转录-聚合酶链反应比较了正常成骨细胞和牙髓干细胞中与骨相关的基因和间充质干细胞标志物的表达水平。两种细胞类型之间差异表达的基因是转录因子 RUNX2、骨桥蛋白（SPP1）、COL1A1（胶原类型 1α1）和碱性磷酸酶（ALPL）。获得的结果表明，CaS 强烈影响 DPSCs 的体外行为，增强了增殖、分化和基质的沉积。
