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锌修饰的钛表面增强牙髓干细胞的体外成骨分化。

Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro.

机构信息

Department of Dentistry, Oral and Maxillofacial-Plastic and Reconstructive Surgery, School of Medicine, Yamagata University, Yamagata, Japan.

Department of Bio-System Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan.

出版信息

Sci Rep. 2016 Jul 8;6:29462. doi: 10.1038/srep29462.

DOI:10.1038/srep29462
PMID:27387130
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4937451/
Abstract

Zinc is an essential trace element that plays an important role in differentiation of osteoblasts and bone modeling. This in vitro study aimed to evaluate the osteoblast differentiation of human dental pulp stem cells (DPSCs) on zinc-modified titanium (Zn-Ti) that releases zinc ions from its surface. Based on real-time PCR, alkaline phosphatase (ALP) activity and Western blot analysis data, we investigated osteoblast differentiation of DPSCs cultured on Zn-Ti and controls. DPSCs cultured on Zn-Ti exhibited significantly up-regulated gene expression levels of osteoblast-related genes of type I collagen (Col I), bone morphogenetic protein 2 (BMP2), ALP, runt-related transcription factor 2 (Runx2), osteopontin (OPN), and vascular endothelial growth factor A (VEGF A), as compared with controls. We also investigated extracellular matrix (ECM) mineralization by Alizarin Red S (ARS) staining and found that Zn-Ti significantly promoted ECM mineralization when compared with controls. These findings suggest that the combination of Zn-Ti and DPSCs provides a novel approach for bone regeneration therapy.

摘要

锌是一种必需的微量元素，在成骨细胞分化和骨建模中发挥重要作用。本体外研究旨在评估锌修饰钛（Zn-Ti）表面释放锌离子对人牙髓干细胞（DPSCs）成骨分化的影响。通过实时 PCR、碱性磷酸酶（ALP）活性和 Western blot 分析数据，我们研究了在 Zn-Ti 和对照条件下培养的 DPSCs 的成骨分化情况。与对照组相比，在 Zn-Ti 上培养的 DPSCs 中与成骨相关的基因Ⅰ型胶原（Col I）、骨形态发生蛋白 2（BMP2）、ALP、 runt 相关转录因子 2（Runx2）、骨桥蛋白（OPN）和血管内皮生长因子 A（VEGF A）的基因表达水平显著上调。我们还通过茜素红 S（ARS）染色研究了细胞外基质（ECM）矿化情况，发现与对照组相比，Zn-Ti 显著促进了 ECM 矿化。这些发现表明，Zn-Ti 与 DPSCs 的结合为骨再生治疗提供了一种新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/98431c730d5d/srep29462-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/a0e1618c1ed2/srep29462-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/ba40a1757ace/srep29462-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/ee2781d74fb2/srep29462-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/ce31d98134fe/srep29462-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/98431c730d5d/srep29462-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/a0e1618c1ed2/srep29462-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/ddff6362c60e/srep29462-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/28521570e390/srep29462-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/8ce123e81206/srep29462-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/ba40a1757ace/srep29462-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/ee2781d74fb2/srep29462-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/ce31d98134fe/srep29462-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5473/4937451/98431c730d5d/srep29462-f8.jpg

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