[可逆性咽鼓管阻塞致豚鼠分泌性中耳炎模型]
[A guinea pigs model of otitis media with effusion caused by reversible Eustachian tube obstruction].
作者信息
Cui Xin-yan, Yu Chen-jie, Chen Feng, Gao Xia
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, Drum Tower Clinical Medical College of Nanjing Medical University, Nanjing 210008, China.
出版信息
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 May;46(5):413-6.
OBJECTIVE
To develop a animal model for acute otitis media with effusion (OME).
METHODS
In 22 guinea pigs, the left nasal orifice of Eustachian tube was approached via a transpalatal incision and obstructed with polyvinyl acetal material. Right ears were set as the control. Then all the ears were evaluated by otomicroscopy every day. Seven, 14 and 21 days after the intervention, six guinea pigs were killed for histologic study.
RESULTS
Of the 22 guinea pigs included in this study, 20 ears (90.9%) were found to have effusion 3 - 7 days after the operation, two cases were excluded for purulent otorrhea 10 days postoperatively. The epithelium initially developed hyperplasia, and the submucosa showed vascular and lymphatic dilatations with inflammatory cells infiltration. None of the contralateral control ears had evidence of disease by otomicroscopic examination and histologic study.
CONCLUSION
This experimental methods provoked reproducible pathologic characteristics similar to those for otitis media with effusion.
目的
建立急性分泌性中耳炎（OME）动物模型。
方法
对22只豚鼠经腭部切口暴露左侧咽鼓管咽口，用聚乙烯醇缩醛材料堵塞。右侧耳朵作为对照。然后每天用耳显微镜对所有耳朵进行评估。干预后7天、14天和21天，处死6只豚鼠进行组织学研究。
结果
本研究纳入的22只豚鼠中，20只耳朵（90.9%）在术后3 - 7天出现积液，2例因术后10天出现脓性耳漏而被排除。上皮最初出现增生，黏膜下层显示血管和淋巴管扩张并有炎性细胞浸润。对侧对照耳经耳显微镜检查和组织学研究均无疾病迹象。
结论
该实验方法引发了与分泌性中耳炎相似的可重复病理特征。
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