2,3,7,8-四氯二苯并对二恶英影响大鼠肝星状细胞和肾脏中视黄醇酯化。

2,3,7,8-Tetrachlorodibenzo-p-dioxin affects retinol esterification in rat hepatic stellate cells and kidney.

机构信息

Institute of Environmental Medicine, Karolinska Institutet, Box 210, S-171 77 Stockholm, Sweden.

出版信息

Environ Toxicol Pharmacol. 1996 Aug 15;2(1):17-23. doi: 10.1016/1382-6689(96)00023-3.

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a highly toxic environmental contaminant, interferes with retinoid homeostasis. To elucidate the underlying mechanism, the activities of lecithin : retinol and acyl-CoA : retinol acyltransferase (LRAT and ARAT) were determined in liver, kidney, and hepatic parenchymal and nonparenchymal cell fractions from rats 7 days after a single oral dose of 10 μg TCDD/kg body weight (b.w). Severely depressed LRAT activity in hepatic stellate cells, and greatly increased LRAT activity in kidneys, as well as decreased ARAT activity in stellate cells, were seen in TCDD-treated rats. Although the relevance of decreased ARAT activity under physiological conditions is not clear, the changed LRAT activities most likely contributes significantly to the TCDD-induced effects on tissue retinyl ester levels. It is intriguing that TCDD affects LRAT activity in hepatic stellate cells and kidney in opposite directions. The results suggest that effects of TCDD on retinyl ester tissue levels could be due to a specific interaction with retinoid metabolism.

摘要

2,3,7,8-四氯二苯并对二恶英（TCDD）是一种高度有毒的环境污染物，会干扰类视黄醇的动态平衡。为了阐明其潜在机制，在大鼠单次口服 10μg TCDD/kg 体重（b.w.）后 7 天，测定了肝脏、肾脏以及肝实质和非实质细胞组分中卵磷脂：视黄醇（LRAT）和酰基辅酶 A：视黄醇酰基转移酶（ARAT）的活性。在 TCDD 处理的大鼠中，肝星状细胞中 LRAT 活性严重降低，而肾脏中 LRAT 活性大大增加，星状细胞中 ARAT 活性降低。尽管在生理条件下 ARAT 活性降低的相关性尚不清楚，但改变的 LRAT 活性很可能对 TCDD 引起的组织视黄醇酯水平的影响有重要贡献。有趣的是，TCDD 以相反的方向影响肝星状细胞和肾脏中的 LRAT 活性。结果表明，TCDD 对视黄醇酯组织水平的影响可能是由于与类视黄醇代谢的特定相互作用。